Protein-protein interactions and glycerophospholipids in bromovirus and nodavirus RNA replication
The plant bromoviruses and animal nodaviruses are distinct groups of positive strand RNA viruses that have proven to be useful models for RNA replication studies. Bromoviruses encode two large proteins required for RNA replication: 1a contains domains implicated in helicase and capping functions, and 2a contains a central polymerase-like domain. Using immunoprecipitation and far-western blotting, we have now shown that la and 2a form a specific complex in vitro and have mapped the interacting domains. Molecular genetic data implicate the 1a–2a complex in RNA replication and suggest that it supports coordinate action of the putative helicase, polymerase, and capping domains. The locations of the interacting la and 2a domains have implications for replication models and the evolution of virus genomes bearing homologous replication genes in fused vs. divided forms. For the nodavirus Flock house virus (FHV), a true RNA replicase has been isolated that carries out complete, highly active replication of added FHV RNA, producing newly synthesized positive strand RNA in pre-dominantly ssRNA form. Positive strand RNA synthesis in this FHV cell-free system is strongly dependent on the addition of any of several glycerophospholipids. Positive strand RNA synthesis depends on the complete glycerophospholipid structure, including the polar head group and diacyl glycerol lipid portion, and is strongly influenced by acyl chain length.
KeywordsPositive Strand Brome Mosaic Virus Cowpea Chlorotic Mottle Virus Flock House Virus dsRNA Product
Unable to display preview. Download preview PDF.
- 7.Francki RIB, Fauquet CM, Knudson DL, Brown F (1991) Classification and nomenclature of viruses: Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York (Arch Virol [Suppl] 2 )Google Scholar
- 8.Goldbach R, LeGall O, Wellink J (1991) Alpha-like viruses in plants. Semin Virol 2: 19–25Google Scholar
- 20.Orr JW, Newton AC (1992) Interaction of protein kinase C with phosphatidylserine. 1. Cooperativity in lipid binding. Biochemistry 31: 4661–4667Google Scholar
- 29.Dinant M, Janda M, Kroner P, Ahlquist P (1993) Bromovirus RNA replication and transcription require compatibility between the polymerase- and helicase-like viral RNA synthesis proteins. J Virol 67 (in press)Google Scholar