Flock house virus: a simple model for studying persistent infection in cultured Drosophila cells

  • R. Dasgupta
  • B. Selling
  • R. Rueckert
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)


Flock house virus (FHV), isolated from twenty Drosophila melanogaster cell lines, persistently infected with the virus, were examined during successive serial passages by plaque assay and sequence analysis. No phenotypic or genotypic changes in the virus were observed during the establishment of persistent infection, suggesting that it was a cellular modification that led to the first step in establishing the persistent state. Once this state was initiated, the virus was relieved of the need for a functional coat protein to propagate itself and mutations began to accumulate selectively in RNA2, the gene for the coat protein. These changes were manifested by a gradual drift to a smaller plaque population. The replicase activity, coded by RNA1, remained unaltered.


Coat Protein Persistent Infection Plaque Assay Plaque Size Drosophila Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Hendry DA (1991) Nodaviridae of invertebrates. In: Kurstak E (ed) Viruses of invertebrates. Marcel Dekker, New York, pp 227–276Google Scholar
  2. 2.
    Selling BH, Allison RF, Kaesberg P (1990) Genomic RNA of an insect virus directs synthesis of infectious virions in plants. Proc Natl Acad Sci USA 87: 434–438PubMedCrossRefGoogle Scholar
  3. 3.
    Ahmed RA, Stevens JG (1989) Viral persistence. In: Fields BN (ed) Virology. Raven Press, New York, pp 241–266Google Scholar
  4. 4.
    Mahy BWJ (1985) Strategies of virus persistence. Br Med Bull 41: 50–55PubMedGoogle Scholar
  5. 5.
    Oldstone MBA (1989) Viral persistence. Cell 56: 517–520PubMedCrossRefGoogle Scholar
  6. 6.
    Ahmed R, Canning WM, Kauffman RS, Sharpe AH, Hallum JV, Fields BN (1981) Role of the host cell in persistent viral infection: coevolution of L cells and reovirus during persistent infection. Cell 25: 325–332PubMedCrossRefGoogle Scholar
  7. 7.
    de la Torre JC, Martinez-Salas E, Diez J, Villverde A, Gebauer F, Rocha E, Davila M, Domingo E (1988) Coevolution of cells in a persistent infection of foot and mouth disease virus in cell culture. J Virol 62: 2050–2058PubMedGoogle Scholar
  8. 8.
    Friesen P, Scotti P, Longworth J, Rueckert R (1980) Black Beetle Virus: Propagation in Drosophila line 1 cells and an infection-resistant subline carrying endogenous Black Beetle Virus-related particles. J Virol 35: 741–747PubMedGoogle Scholar
  9. 9.
    Selling B (1986) Infectivity of Black Beetle Virus in cultured Drosophila cells. Ph.D. thesis, University of Wisconsin-Madison, pp 88–135Google Scholar
  10. 10.
    Dasgupta R, Ghosh A, Dasmahapatra B, Guarino LA, Kaesberg P (1984) Primary and secondary structure of black beetle virus RNA2, the genomic messenger for BBV coat protein. Nucleic Acids Res 12: 7215–7223PubMedCrossRefGoogle Scholar
  11. 11.
    de la Torre JC, de la Luna S, Diez J, Domingo E (1989) Resistance to foot and mouth disease virus mediated by trans-acting cellular products. J Virol 63: 2385–2387PubMedGoogle Scholar
  12. 12.
    Riedel B, Brown DT (1979) Novel antiviral activity found in the media of Sindbis virus persistently infected mosquito (.Aedes albopictus) cell cultures. J Virol 29: 51 - 60PubMedGoogle Scholar
  13. 13.
    Selling BH, Rueckert RR (1984) Plaque assay for black beetle virus. J Virol 51: 251–253PubMedGoogle Scholar
  14. 14.
    Zhong W, Dasgupta R, Rueckert R (1992) Evidence that the packaging signal for nodaviral RNA2 is a bulged stem-loop. Proc Natl Acad Sci USA 89: 11146–11150PubMedCrossRefGoogle Scholar
  15. 15.
    Dasmahapatra B, Dasgupta R, Saunders K, Selling B, Gallagher T, Kaesberg P (1986) Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus. Proc Natl Acad Sei USA 83: 63–66CrossRefGoogle Scholar
  16. 16.
    Kraft R, Tardiff J, Krauter KS, Leinwand L (1988) Using mini-prep plasmid DNA for sequencing double stranded templates with sequenase. Biotechniques 6: 544–549PubMedGoogle Scholar
  17. 17.
    Dasgupta R, Sgro JY (1989) Nucleotide sequence of three nodavirus RNA2’s: the messengers for their coat protein precursors. Nucleic Acids Res 17: 7525–7526PubMedCrossRefGoogle Scholar
  18. 18.
    Devereaux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–395CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • R. Dasgupta
    • 1
  • B. Selling
    • 1
  • R. Rueckert
    • 1
  1. 1.Institute for Molecular Virology, Department of Biochemistry, Graduate School and College of Agriculture and Life SciencesUniversity of WisconsinMadisonUSA

Personalised recommendations