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Flock house virus: a simple model for studying persistent infection in cultured Drosophila cells

  • R. Dasgupta
  • B. Selling
  • R. Rueckert
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)

Summary

Flock house virus (FHV), isolated from twenty Drosophila melanogaster cell lines, persistently infected with the virus, were examined during successive serial passages by plaque assay and sequence analysis. No phenotypic or genotypic changes in the virus were observed during the establishment of persistent infection, suggesting that it was a cellular modification that led to the first step in establishing the persistent state. Once this state was initiated, the virus was relieved of the need for a functional coat protein to propagate itself and mutations began to accumulate selectively in RNA2, the gene for the coat protein. These changes were manifested by a gradual drift to a smaller plaque population. The replicase activity, coded by RNA1, remained unaltered.

Keywords

Coat Protein Persistent Infection Plaque Assay Plaque Size Drosophila Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • R. Dasgupta
    • 1
  • B. Selling
    • 1
  • R. Rueckert
    • 1
  1. 1.Institute for Molecular Virology, Department of Biochemistry, Graduate School and College of Agriculture and Life SciencesUniversity of WisconsinMadisonUSA

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