Monoamine oxidase (MAO; E.C. characteristics of platelets influenced by in vitro and in vivo ethanol on alcoholics and on control subjects

  • T. May
  • H. Rommelspacher
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 41)


Ethanol (ETOH) in vitro displays a competitive inhibition of human platelet MAO-B with a Ki of 270 ± 30 mM. Lineweaver-Burk analyses with 6 substrate concentrations (5-160 μM kynuramine) in the presence or absence of 200 mM ETOH were performed with platelets of alcoholics before withdrawal (Alc day 1), one week (Alc day 8) and 3 months (Alc mon 3) after withdrawal as well as in control subjects without and after ETOH intake.

In all groups the Km increases highly significantly (p < 0.001) but the Vmax is unchanged by the presence of ETOH in vitro supporting the view of a competitive inhibition in each group.

The Vmax of Alc day 1 is significantly 25% decreased, of Alc day 8 unchanged and of Alc mon 3 nonsignificantly 19% decreased in comparison with the controls. The increase of the Km in the presence of 200 mM ETOH is significantly 15% reduced in Alc day 1 and 9% in Alc day 8 but unchanged in Alc mon 3 compared with the controls.


Monoamine Oxidase Human Platelet Stud Alcohol ETOH Intake ETOH Intoxication 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • T. May
    • 1
  • H. Rommelspacher
    • 1
  1. 1.Department of NeuropsychopharmacologyFree UniversityBerlinFederal Republic of Germany

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