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Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding

  • M. C. Anderson
  • K. F. Tipton
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 41)

Summary

A modification of the [3H]-pargyline labelling technique is presented for determining the active-site concentration of monoamine oxidase in soluble preparations. Kinetic considerations show that the rate of reaction of MAO-A with low concentrations of free pargyline will be very much slower than that of MAO-B. Failure to use adequate reaction times for the concentration of pargyline added can lead to gross underestimation of the quantity of MAO-A present.

Keywords

Monoamine Oxidase Enzyme Sample Resuspend Pellet Complete Labelling Soluble Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Fowler CJ, Mantle TJ, Tipton KF (1982) The nature of the inhibition of rat liver monoamine oxidase types A and B by the acetylenic inhibitors clorgyline, l-deprenyl and pargyline. Biochem Pharmacol 31: 2561–3555.CrossRefGoogle Scholar
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. C. Anderson
    • 1
  • K. F. Tipton
    • 1
  1. 1.Department of BiochemistryTrinity CollegeDublin 2Ireland

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