Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding

  • M. C. Anderson
  • K. F. Tipton
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 41)


A modification of the [3H]-pargyline labelling technique is presented for determining the active-site concentration of monoamine oxidase in soluble preparations. Kinetic considerations show that the rate of reaction of MAO-A with low concentrations of free pargyline will be very much slower than that of MAO-B. Failure to use adequate reaction times for the concentration of pargyline added can lead to gross underestimation of the quantity of MAO-A present.


Monoamine Oxidase Enzyme Sample Resuspend Pellet Complete Labelling Soluble Preparation 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. C. Anderson
    • 1
  • K. F. Tipton
    • 1
  1. 1.Department of BiochemistryTrinity CollegeDublin 2Ireland

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