Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding
A modification of the [3H]-pargyline labelling technique is presented for determining the active-site concentration of monoamine oxidase in soluble preparations. Kinetic considerations show that the rate of reaction of MAO-A with low concentrations of free pargyline will be very much slower than that of MAO-B. Failure to use adequate reaction times for the concentration of pargyline added can lead to gross underestimation of the quantity of MAO-A present.
KeywordsMonoamine Oxidase Enzyme Sample Resuspend Pellet Complete Labelling Soluble Preparation
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- Tipton KF (1985) Determination of monoamine oxidase. Meth Find Exp Clin Pharmacol 7: 361–367.Google Scholar