Advertisement

Several aspects on the amine oxidation by semicarbazide-sensitive amine oxidase (SSAO) from bovine lung

  • J. M. Lizcano
  • A. Fernandez de Arriba
  • G. A. Lyles
  • M. Unzeta
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 41)

Summary

The lung has been shown to be potentially important in the metabolism of amines. Since SSAO has been demonstrated to be active towards some volatile short-chain aliphatic amines in other tissues, the current study determined the specificity and kinetic constants for the metabolism by bovine lung SSAO, of several aliphatic and aromatic amines some of which have been suggested to be physiological substrates (e.g. methylamine, aminoacetone and ß-phenylethylamine), and others (e.g. benzylamine) which are non-physiological. In the case of benzylamine, an inhibition at high substrate concentration was observed. Kinetic assays ruled out the possibility that this inhibition was caused by products of the de-amination of benzylamine, and consequently it is suggested that these results may indicate the presence of two binding sites for the interaction of benzylamine with SSAO.

Keywords

Benzyl Alcohol Amine Oxidase High Substrate Concentration Bovine Lung SSAO Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Dixon M, Webb EC, Thorne CJR, Tipton KF (1979) Enzymes, 3rd edn. Longman, London, pp 126–136.Google Scholar
  2. Fowler CJ, Tipton KF (1981) Concentration dependence of the oxidation of tyramine by the two forms of rat liver mitochondrial monoamine oxidase. Biochem Pharmacol 30: 3329–3332.PubMedCrossRefGoogle Scholar
  3. Friedenwald JS, Maengwyn-Davies GD, McElroy WD, Glass B (eds) (1954) The mechanism of enzyme action. The Johns Hopkins Press, Baltimore, p 180.Google Scholar
  4. Lewinsohn R (1984) Mammalian monoamine oxidizing enzymes with special reference to benzylamine oxidase in human tissues. Brazilian J Med Biol Res 17: 223–256.Google Scholar
  5. Lizcano JM, Balsa D, Tipton KF, Unzeta M (1990) Amine oxidase activities in bovine lung. J Neural Transm [Suppl] 32: 341–344.Google Scholar
  6. Lyles GA, Singh I (1985) Vascular smooth muscle cells: a major source of the semi-carbazide-sensitive amine oxidase of the rat aorta. J Pharm Pharmacol 37: 637–647.PubMedCrossRefGoogle Scholar
  7. Lyles GA, Holt A, Marshall S (1990) Further studies on the metabolism of methyla-mine by semicarbazide-sensitive amine oxidase in human plasma, umbilical artery and rat aorta. J Pharm Pharmacol 42: 332–338.PubMedCrossRefGoogle Scholar
  8. Lyles GA, Chalmers J (1992) The metabolism of aminoacetone to methylglyoxal by semicarbazide-sensitive amine oxidase in human umbilical artery. Biochem Pharmacol 43: 1409–1414.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. M. Lizcano
    • 1
  • A. Fernandez de Arriba
    • 1
  • G. A. Lyles
    • 2
  • M. Unzeta
    • 1
  1. 1.Departament de Bioquimica i Biologia Molecular, Facultat de MedicinaUniversitat Autonoma de BarcelonaBellaterra (Barcelona)Spain
  2. 2.Department of Pharmacology and Clinical Pharmacology, Ninewells Hospital and Medical SchoolUniversity of DundeeDundeeUK

Personalised recommendations