Reactive gliosis and monoamine oxidase B

  • J. Ekblom
  • S. S. Jossan
  • L. Oreland
  • E. Walum
  • S.-M. Aquilonius
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 41)


A double-staining method was applied to cryosections of human spinal cord from patients who died with amyotrophic lateral sclerosis (ALS) and corresponding controls in order to investigate cellular content of monoamine oxidase B (MAO-B). 3H-L-Deprenyl emulsion autoradiography was used in combination with histochemical methods for the detection of astrocytes and monocytes/microglia. In the ALS spinal cords an increased number of astrocytes as well as an increased content of MAO-B in reactive species of astrocytes was demonstrated. No significant 3H-L-deprenyl binding was observed in cells derived from the mesoderm, e.g. monocytes or microglia. Furthermore, a sub-population of reactive astrocytes that contained low levels of MAO-B was observed in spinal sections. These findings were further substantiated by studies performed on primary astrocyte cultures.


Amyotrophic Lateral Sclerosis Glial Fibrillary Acidic Protein Monoamine Oxidase Reactive Astrocyte Quinolinic Acid 
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  1. Aquilonius S-M, Jossan SS, Ekblom J, Askmark H, Gillberg P-G (1992) Increased binding of L-deprenyl in spinal cords from patients with amyotrophic lateral sclerosis as demonstrated by autoradiography. J Neural Transm [Gen Sect] 89: 111–122.CrossRefGoogle Scholar
  2. Bignami A, Eng LF, Dahl D, Uyeda CT (1972) Localization of the glial fibrillary acidic protein in astrocytes. Brain Res 43: 429–435.PubMedCrossRefGoogle Scholar
  3. Bonduelle M (1975) Amyotrophic lateral sclerosis. In: Vinken PJ, Bruyn GW (eds) Handbook of neurology, vol 22. Elsevier, Amsterdam, pp 281–338.Google Scholar
  4. Cohen G (1983) The pathobiology of Parkinson’s disease: biochemical aspects of neuron senescence. J Neural Transm 19: 89–103.Google Scholar
  5. Diedrich JF, Minnigan H, Carp RI, Whitaker JN, Race R, Freyll W, Haase AT (1991) Neuropathological changes in scrapie and Alzheimer’s disease are associated with increased expression of apolipoprotein E and cathepsin D in astrocytes. J Virol 65: 4759–4768.PubMedGoogle Scholar
  6. Ekblom J, Jossan SS, Bergström M, Oreland L, Walum E, Aquilonius S-M (1993) Monoamine oxidase-B in astrocytes. Glia 8: 122–132.PubMedCrossRefGoogle Scholar
  7. Fedoroff S, Neal J, Opas M, Kalnis V (1984) Astrocyte cell lineage. III. The morphology of differentiating mouse astrocytes in colony culture. J Neurocytol 13: 1–20.PubMedCrossRefGoogle Scholar
  8. Gillberg P-G, Jossan SS, Askmark H, Aquilonius S-M (1986) Large-section cryo-microtomy for in vitro receptor autoradiography. J Pharmacol Toxicol Methods 15: 169–180.Google Scholar
  9. Jossan SS, Gillberg P-G, Gottfries C-G, Karlsson I, Oreland L (1991) Monoamine oxidase-B in brains from patients with Alzheimer’s disease: a biochemical and autoradiographical study. Neuroscience 45: 1–12.PubMedCrossRefGoogle Scholar
  10. Levitt P, Pintar JE, Breakfield XO (1982) Immunohistochemical demonstration of monoamine oxidase B in brain astrocytes and serotonergic neurons. Proc Natl Acad Sci USA 79: 6385–6389.PubMedCrossRefGoogle Scholar
  11. Magyar K, Knoll J (1977) Selective inhibition of the B-form of monoamine oxidase. Pol J Pharmacol 29: 233–246.Google Scholar
  12. Mannoji H, Yeger J, Becker LF (1986) A specific histochemical marker (lectinRicinus communis agglutinin-1) for normal human microglia and application to routine histopathology. Acta Neuropathol 71: 341–343.PubMedCrossRefGoogle Scholar
  13. McKeon RJ, Schreiber RC, Rudge JS, Silver J (1991) Reduction of neurite outgrowth in a model of glial scarring following CNS injury is correlated with the expression of inhibitory molecules on reactive astrocytes. J Neurosci 11: 3398–3411.PubMedGoogle Scholar
  14. Nakamura S, Kawamata T, Akiguchi I, Kameyama M, Nakamura N, Kimuara H (1990) Expression of monoamine oxidase B activity in astrocytes of senile plaques. Acta Neuropathol 86: 419–425.CrossRefGoogle Scholar
  15. Schoepp DD, Azzaro AJ (1983) Effects of intrastriatal kainic acid injections on 3H-dopamine metabolism in rat striatal slices: evidence for postsynaptic glial metabolism by both type A and B forms of monoamine oxidase. J Neurochem 40: 1340–1343.PubMedCrossRefGoogle Scholar
  16. Schwarcz R (1992) Endogeneous excitotoxins in neurodegenerative brain diseases. Behav Pharmacol 13: 25.CrossRefGoogle Scholar
  17. Strolin Benedetti M, Dostert P (1989) Monoamine oxidase, brain ageing and degenerative diseases. Biochem Pharmacol 38: 555–561.CrossRefGoogle Scholar
  18. Tanaka H, Araki M, Masuzawa T (1991) Differential response of three astrocyte-specific proteins to fimbrial transection of the rat brain: immunohistochemical observations with antibodies against glial fibrillary acidic protein, glutamine syn-thetase and S-100 protein. Acta Histochem Cytochem 24: 11–19.CrossRefGoogle Scholar
  19. Walum E, Hansson E, Harvey AL (1991) In vitro testing of neurotoxicity. ATLA 18: 153–179.Google Scholar
  20. Westlund KL, Denney RM, Kocherbergre LM, Rose RM, Abell CW (1985) Distinct monoamine oxidase A and B populations in primate brain. Science 230: 181–183.PubMedCrossRefGoogle Scholar
  21. Westlund KL, Denney RM, Rose RM, Abell CW (1988) Localisation of distinct monoamine oxidase A and monoamine oxidase B cell populations in human brain stem. Neuroscience 25: 439–456.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. Ekblom
    • 1
  • S. S. Jossan
    • 2
  • L. Oreland
    • 2
  • E. Walum
    • 3
  • S.-M. Aquilonius
    • 1
  1. 1.Department of NeurologyUniversity HospitalUppsalaSweden
  2. 2.Department of Medical PharmacologyBiomedical CentreUppsalaSweden
  3. 3.Department of Neurochemistry and NeurotoxicologyUniversity of StockholmStockholmSweden

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