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Iron and neurotoxin intoxication: comparative in vitro and in vivo studies

  • W. Wesemann
  • St. Blaschke
  • H.-W. Clement
  • Chr. Grote
  • N. Weiner
  • W. Kolasiewicz
  • K.-H. Sontag
Part of the Key Topics in Brain Research book series (KEYTOPICS)

Summary

The effect of iron on rat brain was studied in vivo in the nigrostriatal system and in vitro by assay of the lipid peroxidation in membranes isolated from cortex, hippocampus, and striatum. After intranigral injection of Fe (III) a reduced dopamine (DA) metabolism was observed in the striatum by in vivo pulse voltammetry. A retrograde lesion of the substantia nigra by intrastriatal injection of 6-hydroxydopamine (6-OHDA) could be enhanced by co-injection of iron. The lipid peroxidation as measured by ascorbate-induced malondialdehyde (MDA) production was increased by incubation with iron, whereas the iron chelator deferoxamine inhibited the formation of MDA.

Keywords

Lipid Peroxidation Substantia Nigra Differential Pulse Voltammetry Intact Side Intrastriatal Injection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 1993

Authors and Affiliations

  • W. Wesemann
    • 1
  • St. Blaschke
    • 1
  • H.-W. Clement
    • 1
  • Chr. Grote
    • 1
  • N. Weiner
    • 1
  • W. Kolasiewicz
    • 2
  • K.-H. Sontag
    • 2
  1. 1.Institute of Physiological Chemistry, Department of NeurochemistryPhilipps-UniversityMarburgGermany
  2. 2.Max-Planck-Institute of Experimental MedicineGöttingenFederal Republic of Germany

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