Iron and neurotoxin intoxication: comparative in vitro and in vivo studies
The effect of iron on rat brain was studied in vivo in the nigrostriatal system and in vitro by assay of the lipid peroxidation in membranes isolated from cortex, hippocampus, and striatum. After intranigral injection of Fe (III) a reduced dopamine (DA) metabolism was observed in the striatum by in vivo pulse voltammetry. A retrograde lesion of the substantia nigra by intrastriatal injection of 6-hydroxydopamine (6-OHDA) could be enhanced by co-injection of iron. The lipid peroxidation as measured by ascorbate-induced malondialdehyde (MDA) production was increased by incubation with iron, whereas the iron chelator deferoxamine inhibited the formation of MDA.
KeywordsLipid Peroxidation Substantia Nigra Differential Pulse Voltammetry Intact Side Intrastriatal Injection
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