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Accumulation of a cellular protein bearing c-myc-like antigenicity in hepatic and non-hepatic delta antigen expressing cells

  • G. Tappero
  • G. Natoli
  • F. Negro
  • Antonina Smedile
  • F. Bonino
  • M. Rizzetto
  • M. Levrero
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 8)

Summary

Patients with chronic but not acute hepatitis delta virus infection undergo a strong accumulation of a protein of cellular origin which specifically reacts with a panel of anti-c-myc antibodies and which is expressed in the same nuclei that express the delta antigen. In this paper we report on the in vitro characterization of this phenomenon. The delta antigen induced c-myc antigen accumulation can occur in vitro upon transfection of HBsAg positive and negative cell lines with HDAg expression vectors. Using recombinant vaccinia viruses expressing only p24 or p27 we demonstrate that structures common to the two isoforms of HDAg are responsible for the phenomenon, which is not restricted to cells of hepatic origin.

Keywords

HepG2 Cell Hepatitis Delta Virus Recombinant Vaccinia Virus Hepatitis Delta Virus Infection Delta Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • G. Tappero
    • 1
  • G. Natoli
    • 2
  • F. Negro
    • 3
  • Antonina Smedile
    • 3
  • F. Bonino
    • 3
  • M. Rizzetto
    • 4
  • M. Levrero
    • 2
  1. 1.Clinica Medica GeneraleOsp. San Luigi OrbassanoTorinoItaly
  2. 2.I Clinica Medica and Fondazione Andrea CesalpinoUniversita’ La SapienzaRomeItaly
  3. 3.Divisione di GastroenterologiaOsp. Le MolinetteTorinoItaly
  4. 4.Istituto di Medicina InternaUniversita di TorinoTorinoItaly

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