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Detection of replicative intermediates of viral RNA in peripheral blood mononuclear cells from chronic hepatitis C virus carriers

  • M. Artini
  • G. Natoli
  • Maria Laura Avantaggiati
  • Clara Balsano
  • P. Chirillo
  • A. Costanzo
  • Maria Simona Bonavita
  • M. Levrero
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 8)

Summary

Clinical and experimental evidence suggests the possible existence of one or more extrahepatic sites of HCV infection. In order to demonstrate the “in vivo” infection of lymphoid cells by HCV, we applied a nested PCR to total cytoplasmic RNA extracted from fresh or cultured peripheral blood mononuclear cells (PBMCs) of HCV chronically infected patients, using primers derived from the highly conserved 5′ untranslated region of the HCV genome. The presence of virions in PBMCs occurs frequently, if not always, and is often accompanied by active viral replication. Moreover, the appearance of replicative intermediates after stimulation of cellular growth with mitogens suggests that latent genomes could undergo replication upon cellular activation and/or proliferation.

Keywords

Chronic Active Hepatitis Phorbol Myristate Acetate Replicative Intermediate Chronic Persistent Hepatitis Active Viral Replication 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. Artini
    • 1
  • G. Natoli
    • 1
  • Maria Laura Avantaggiati
    • 1
  • Clara Balsano
    • 1
  • P. Chirillo
    • 1
  • A. Costanzo
    • 1
  • Maria Simona Bonavita
    • 1
  • M. Levrero
    • 1
  1. 1.I Clinica Medica and Fondazione Andrea CesalpinoUniversita’ La SapienzaRomeItaly

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