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Isolate antibody to hepatitis C virus core antigen (C22) by RIBA-2: correlation with HCV-RNA and anti-NS5

  • G. Taliani
  • M. C. Badolato
  • R. Lecce
  • R. Bruni
  • C. Clementi
  • F. Grimaldi
  • C. Furlan
  • M. Manganaro
  • F. Duca
  • A. Bozza
  • G. Poliandri
  • C. De Bac
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 8)

Summary

The presence of circulating hepatitis C virus genome (HCV- RNA), elevated ALT levels and antibodies to an NS5-derived synthetic peptide have been examined in 13 subjects with isolate positivity for antibodies to the HCV core antigen (C22) on RIBA-2 testing. All subjects were followed up for 8–18 months (mean 12.4 months). In seven subjects (54%), intermittent or persistent viremia was associated with abnormal ALT levels (6 subjects) and with positivity for antibodies to NS5-peptide (6 subjects). On the other hand, in 6 out of 13 subjects (46%) no viral replication, no liver cytonecrosis and no antibodies to NS5 were found. It is concluded that isolate reactivity to C22 by RIBA-2 is a heterogeneous condition that corresponds to two distinct categories of subjects: those with active HCV infection and those without evidence of virus replication. Although HCV-RNA determination is the most reliable means of identifying HCV carriers, antibodies to NS5 can be a useful marker of virus activity. In fact, antibodies to NS5 were detected in 6 out of 7 viremic patients, compared to 0 out of 6 non-viremic patients (P = 0.004). It remains to be elucidated whether the isolate reactivity to core antigen found in non-viremic subjects represents a specific, HCV- induced antibody response, or is an unrelated crossreactivity.

Keywords

Core Antigen Isolate Positivity Core Peptide Positive Serum Sample Viremic Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • G. Taliani
    • 1
  • M. C. Badolato
    • 1
  • R. Lecce
    • 1
  • R. Bruni
    • 1
  • C. Clementi
    • 1
  • F. Grimaldi
    • 1
  • C. Furlan
    • 1
  • M. Manganaro
    • 1
  • F. Duca
    • 1
  • A. Bozza
    • 1
  • G. Poliandri
    • 1
  • C. De Bac
    • 1
  1. 1.Institute of Tropical Disease, Policlinico Umberto I“La Sapienza” UniversityRomeItaly

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