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Chronic infection in woodchucks infected by a cloned hepatitis delta virus

  • Anna R. Ciccaglione
  • Maria Rapicetta
  • Antonia Fabiano
  • C. Argentini
  • Maria Silvestro
  • R. Giuseppetti
  • F. Varano
  • Nicoletta D’Urso
  • L. Dinolfo
  • Anna Morgando
  • R. Bruni
  • A. Ponzetto
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 8)

Summary

Two woodchucks (Marmota monax) intrahepatically inoculated with hepatitis delta virus (HDV) complementary DNA clones pSVL-D3 and pSVL-Ag showed virological and pathological signs of acute and chronic HDV infection. HDV-RNA and hepatitis delta antigen (HDAg) were detected in serum by slot-blot hybridization and by western blot five weeks after inoculation. Liver biopsy specimens collected at 8th week post inoculum were positive for HDV-RNA. Anti-HDV antibodies were detected at the 11th and 9th weeks, respectively. Histological finding of hepatocarcinoma and persistence of circulating HDV-RNA and anti-HDV were observed up to the 10th month. Both woodchucks produced “small” and “large” HDAg antigen, although the inoculated cloned DNA bears the coding capability solely for the small antigen. A transient decrease of woodchuck hepatitis virus DNA (WHV-DNA) level was observed during the peak of HDV infection. Successive inoculation of acute-phase serum in three woodchucks resulted in a successful infection in one of the animals.

Keywords

Hepatitis Delta Virus DEAE Dextran Woodchuck Hepatitis Virus Immunoblot Procedure Hepatitis Delta Virus Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Anna R. Ciccaglione
    • 1
  • Maria Rapicetta
    • 1
  • Antonia Fabiano
    • 2
  • C. Argentini
    • 1
  • Maria Silvestro
    • 2
  • R. Giuseppetti
    • 1
  • F. Varano
    • 1
  • Nicoletta D’Urso
    • 2
  • L. Dinolfo
    • 2
  • Anna Morgando
    • 2
  • R. Bruni
    • 1
  • A. Ponzetto
    • 2
  1. 1.Department of VirologyIstituto Superiore di SanitàRomeItaly
  2. 2.Department of GastroenterologyOspedale Le MolinetteTorinoItaly

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