Summary
Two woodchucks (Marmota monax) intrahepatically inoculated with hepatitis delta virus (HDV) complementary DNA clones pSVL-D3 and pSVL-Ag showed virological and pathological signs of acute and chronic HDV infection. HDV-RNA and hepatitis delta antigen (HDAg) were detected in serum by slot-blot hybridization and by western blot five weeks after inoculation. Liver biopsy specimens collected at 8th week post inoculum were positive for HDV-RNA. Anti-HDV antibodies were detected at the 11th and 9th weeks, respectively. Histological finding of hepatocarcinoma and persistence of circulating HDV-RNA and anti-HDV were observed up to the 10th month. Both woodchucks produced “small” and “large” HDAg antigen, although the inoculated cloned DNA bears the coding capability solely for the small antigen. A transient decrease of woodchuck hepatitis virus DNA (WHV-DNA) level was observed during the peak of HDV infection. Successive inoculation of acute-phase serum in three woodchucks resulted in a successful infection in one of the animals.
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References
Glenn JS, Taylor J, White JM (1990) In vitro-synthesis of hepatitis delta virus RNA initiates genome replication in cultured cells. J Virol 64: 3104–3107
Kuo MY, Chao M, Taylor J (1989) Initiation of replication of the human hepatitis delta virus genome cloned DNA: role of delta antigen. J Virol 63: 1945–1950
Ponzetto A (1991) Hepatitis delta virus: a clinical outlook. In: Hollinger FB, Lemon SM, Margolis H (eds) Viral hepatitis and liver disease. Wilkins, Baltimore, pp 882–884
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
Sureau C, Taylor J, Chao M, Eichberg JW, Lanford RE (1989) Cloned hepatitis delta virus cDNA is infectious in the chimpanzee. J Virol 63: 4292–4297
Zignego AL, Deny P, Feray C, Ponzetto A, Gentilini P, Tiollais P, Brechot C (1990) Amplification of hepatitis delta virus RNA sequences by polymerase chain reaction: a tool for viral detection and cloning. Mol Cell Probes 4: 43–51
Zyzik E, Ponzetto A, Forzani B, Hele C, Heermann KH, Gerlich WH (1987) Proteins of Hepatitis delta virus in serum and liver. In: Robinson W, Koike K, Will H (eds) Hepadnaviruses. Alan R. Liss, New York, pp 565–577
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© 1993 Springer-Verlag
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Ciccaglione, A.R. et al. (1993). Chronic infection in woodchucks infected by a cloned hepatitis delta virus. In: Gerlich, W.H. (eds) Research in Chronic Viral Hepatitis. Archives of Virology Supplementum, vol 8. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9312-9_2
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DOI: https://doi.org/10.1007/978-3-7091-9312-9_2
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82497-9
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