Detection of hepatitis B virus DNA by polymerase chain reaction in vaccinated and non-vaccinated Senegalese children

  • M. Chabaud
  • N. Depril
  • P. Le Cann
  • D. Leboulleux
  • R. Nandi
  • A-M. Coll-Seck
  • P. Coursaget
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 8)


An hepatitis B immunization programme was initiated in Senegal in 1978, and infants included in this controlled study have been followed for a period of 2–12 years after immunization. During this period HBV infections have been observed both in vaccinated and non-vaccinated infants. The polymerase chain reaction was used to search for HBV DNA sequences in the sera of 153 children with evidence of serum markers of past or present HBV replication. Amplified HBV DNA sequences were detected in 93% of the HBsAg positive individuals, in 58% of those only positive for antiHBc antibodies and in 7.8% of antiHBs and antiHBc positive infants. The results confirm the high efficiency and long-lasting effectiveness of HB vaccine.


HBsAg Carrier HBsAg Positive Individual Thiers Versus Senegalese Child 
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  1. 1.
    Beasley RP (1982) Hepatitis B virus as the etiologic agent in hepatocellular carcinoma-epidemiologic considerations. Hepatology 2: 21–26Google Scholar
  2. 2.
    Blum HE, Liang TJ, Galun E, Wands JR (1991) Persistence of hepatitis B viral DNA after serological recovery from hepatitis B virus infection. Hepatology 14: 56–62PubMedCrossRefGoogle Scholar
  3. 3.
    Bréchot C, Degos F, Lugassy C, Thiers V, Zafrani S, Franco D, Bismuth H, Trépo C, Benhamou JP, Wands J, Isselbacher K, Tiollais P, Berthelot P (1985) Hepatitis B virus DNA in patients with chronic liver disease and negative tests for hepatitis B surface antigen. N Engl J Med 321: 270–276CrossRefGoogle Scholar
  4. 4.
    Bréchot C, Kemsdorf D, Paterlini P, Thiers V (1991) Hepatitis B virus DNA in HBsAg-negative patients. J Hepatol 13: S49–S35PubMedCrossRefGoogle Scholar
  5. 5.
    Chemin I, Baginski MA, Petit MA, Zoulim F, Pichoud C, Caped F, Hantz O, Trépo C (1991) Correlation between HBV DNA detection by polymerase chain reaction and pre-S1 antigenemia in symptomatic and asymptomatic hepatitis B virus infections. J Med Virol 33: 51–57PubMedCrossRefGoogle Scholar
  6. 6.
    Coursaget P, Yvonnet B, Chotard J, Sarr M, Vincelot P, N’Doye R, Diop-Mar I, Chiron JP (1986) Seven years study of hepatitis B vaccine efficacy in infants from an endemic area ( Senegal ). Lancet ii: 1143–1145Google Scholar
  7. 7.
    Coursaget P, Le Cann P, Leboulleux D, Diop-Mar I, Bao O, Coll AM (1991) Detection of hepatitis B virus DNA by polymerase chain reaction in HBsAg negative Senegalese patients suffering from cirrhosis or primary liver cancer. FEMS Microbiol Lett 83: 35–38CrossRefGoogle Scholar
  8. 8.
    Coursaget P, Leboulleux D, Soumare M, Le Cann P, Yvonnet B, Chiron JP, Coll- Secka M, Diop-Mar I (1993) Twelve-year follow-up study of hepatitis B vaccination of Senegalese infants. J Hepatol (in press)Google Scholar
  9. 9.
    Diamantis ID, Mc Gandy C, Pult I, Bühler H, Schmid M, Gudat F, Bianchi L (1992) Polymerase chain reaction detects hepatitis B virus DNA in paraffin-embedded liver tissue from patients sero and histo-negative for active hepatitis B. Virchows Archiv A Pathol Anat Histopathol 420: 11–15CrossRefGoogle Scholar
  10. 10.
    Fortuin M, Karthigesu V, Allison L, Howard C, Hoare S, Mendy M, Whittle H (1993) Break-through infections and identification of a viral mutant in Gambian children immunised with hepatitis B vaccine (submitted)Google Scholar
  11. 11.
    Hadler SC, Francis DP, Maynard JE, Thompson SE, Juobon FN, Echenberg DF, Ostrow DG, O’Malley PM, Penley KA, Altman NL (1986) Long term immunogenicity and efficacy of hepatitis B vaccine in homosexual men. N Engl J Med 315: 209–214PubMedCrossRefGoogle Scholar
  12. 12.
    Katychaki JN, Siem TH, Brouver R (1978) Serological evidence of presence of HBsAg undetectable by conventional radioimmunoassay in anti-HBc positive blood donnors. J Clin Pathol 31: 837–839CrossRefGoogle Scholar
  13. 13.
    Kuhns M, McNamara A, Mason A, Campbell C, Perrillo R (1992) Serum and liver hepatitis B virus DNA in chronic hepatitis B after sustained loss of surface antigen. Gastroenterology 103: 1649–1656PubMedGoogle Scholar
  14. 14.
    Lai ME, Farci P, Figus A, Balestrieri A, Arnone M, Vyas GN (1989) Hepatitis B virus DNA in the serum of Sardinian blood donors negative for the hepatitis B surface antigen. Blood 73: 17–18 /30Google Scholar
  15. 15.
    Lee HS, Ulrich PP, Vyas GN (1991) Mutations in S gene affecting the immunologic determinants of the envelope protein of hepatitis B virus. J Hepatol 13: S97–S101PubMedCrossRefGoogle Scholar
  16. 16.
    Luo KX, Zhou R, He C, Liang ZS, Jiang S (1991) Hepatitis B virus DNA in sera of virus carriers positive exclusively for antibodies to the hepatitis B core antigen. J Med Virol 35: 55–59PubMedCrossRefGoogle Scholar
  17. 17.
    Maupas P, Coursaget P, Chiron JP, Goudeau A, Barin F, Perrin J, Denis F, Diop-Mar I (1981a) Active immunization against hepatitis B in an aera of high endemicity. Part I: field design. Prog Med Virol 27: 168–184PubMedGoogle Scholar
  18. 18.
    Maupas P, Chiron JP, Barin F, Coursaget P, Goudeau A, Perrin J, Denis F, Diop-Mar I (1981b) Efficacity of hepatitis B vaccine in prevention of early HBsAg carrier state in children controlled trial in an endemic area ( Senegal ). Lancet i: 289–292Google Scholar
  19. 19.
    Paterlini P, Gerken G, Nakajima E, Terre S, D’Errico A, Grigioni W, Franco D, Nalpas B, Wands J, Kew M, Pisi E, Tiollais P, Brechot C (1990) Polymerase chain reaction to detect hepatitis B virus DNA and RNA sequences in primary liver cancers from patients negative for hepatitis B surface antigen. N Engl J Med 323: 80–85PubMedCrossRefGoogle Scholar
  20. 20.
    Shafritz DA, Lieberman HM, Isselbacher KJ, Wands JR (1982) Monoclonal radio-immunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes. Proc Natl Acad Sci USA 79: 5675–5679PubMedCrossRefGoogle Scholar
  21. 21.
    Shih LN, Sheu JC, Wang JT, Huang GI, Yang PM, Lee HS, Sung JL, Wang TH, Chen DS (1990) Serum hepatitis B virus DNA in healthy HBsAg negative Chinese adults evaluated by polymerase chain reaction. J Med Virol 32: 257–260PubMedCrossRefGoogle Scholar
  22. 22.
    Thiers V, Nakajima E, Kremsdorf K, Mack K, Schellekens H, Driss F, Goudeau A, Wands J, Sninsky J, Tiollais P, Brechot C (1988) Transmission of hepatitis B from hepatitis B seronegative subjects. Lancet ii: 1273–1276Google Scholar
  23. 23.
    Wands JR, Bruns RR, Carlson RI, Ware A, Menitove JE, Isselbacher KJ (1982) Monoclonal IgM radioimmunoassay for hepatitis B surface antigen: high binding activity in serum that is unreactive with conventional antibodies. Proc Natl Acad Sci USA 79: 1277–1281PubMedCrossRefGoogle Scholar
  24. 24.
    Wang JT, Wang TH, Sheu JC, Shih LN, Lin JT, Chen DS (1990) Detection of Hepatitis B virus DNA by polymerase chain reaction in plasma volunteer blood donors negative for hepatitis B surface antigen. J Infect Dis 163: 397–399CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. Chabaud
    • 1
  • N. Depril
    • 1
  • P. Le Cann
    • 1
  • D. Leboulleux
    • 2
  • R. Nandi
    • 1
  • A-M. Coll-Seck
    • 2
  • P. Coursaget
    • 1
  1. 1.Institut de Virologie de ToursTours CedexFrance
  2. 2.Faculté de Médecine et PharmacieService des Maladies InfectieusesDakarSénégal

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