Serological and antigenical findings indicating pestivirus in man
An epidemiological survey for pestivirus was undertaken in Zambia and Europe, in view of the recent serological findings obtained by previous studies in Europe with humans. Collected sera were tested for anti-bovine viral diarrhea virus (BVDV) specific antibodies by IIF and Western Blotting. Of those individuals tested (n = 1272), 15.3% showed a seropositive reaction to the BVDV. Anti-BVDV antibody prevalence in immuno-depressed patients (e.g. HIV positive) was investigated. A higher prevalence was revealed in HIV patients suffering from chronic diarrhoea and in those having developed AIDS Related Complex (ARC). Out of 212 persons tested for pestivirus isolation, a non cytopathic virus strain was detected in 2 buffy coat samples using IIF with a specific anti-BVDV serum. The isolation could be repeated three times during 31 days in one person. The virus was identified as a pestivirus with radioimmuno-precipitation assays and IIF-flow cytometry. A doublet of 120 kD was identified only in cell lysates, indicating a non-structural protein. In order to rule out cross reactivity 30 sera from Hepatitis C seropositive patients were tested against the isolate by IIF-flow cytometry. No antigen-specific binding could be observed. These findings indicated the occurrence of a pestivirus in man and might suggest a relationship with a pestivirus of animal origin.
KeywordsHuman Immunodeficiency Virus Human Immunodeficiency Virus Infection West Nile Virus Bovine Viral Diarrhoea Virus Human Immunodeficiency Virus Patient
Unable to display preview. Download preview PDF.
- 1.Bolin SR, McClurkin AW, Coria MF (1985) Frequency of persistent BVDV infection in selected cattle herds. Am J Vet Res 46: 2378–2385Google Scholar
- 3.Castrucci G (1978) Togaviridae. In: Infezione da virus degli animali domestici. Edagricolae, Milan 3: 114–201Google Scholar
- 5.Franki RIB, Fauquet CM, Knudson DL, Braun F (eds) (1991) Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York (Arch Virol [Suppl] 2 )Google Scholar
- 6.Giangaspero M (1986) Infezione da virus BVD/Recettivit umana al BVD. Thesis of Doctorat. University of Milan, ItalyGoogle Scholar
- 7.Giangaspero M (1989) La Diarrhee Virale des Bovins — BVD. Detection d’anticorps specifiques chez l’homme. Thesis of MSc 8 IMT Antwerp BelgiumGoogle Scholar
- 8.Gianpaspero M, Blondeel H, Bear J, Alders R, Morgan D (1991) Epidemiological survey on Bovine Viral Diarrhoea virus in Zambian cattle. Zimbabwe Vet J 22: 57–63Google Scholar
- 10.Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227: 680–686Google Scholar
- 15.Stoeber M (1982) Streptotrichose der Haut (Dermatophytose) bei Mastbullen mit BVD. Tieraerztl Umschau 37: 629–630Google Scholar
- 17.Vacirca G, Giangaspero M, Belloli A, Bertoli G, Citrino A (1988) Ricerca di Anticorpi anti-BVD in siero di sangue umano. Meeting 8: 37–39Google Scholar
- 18.Wellemans G, Leunen J (1973) La rhinotracheite infectieuse des bovins ( IBR) et sa serologie. Ann Med Vet 117: 507–511Google Scholar
- 19.Wellemans G, Vanopdenbosch E (1990) Depistage des bovins BVD-immunotolerants (BVD-IT) a partir des lymphocytes: un apercu de 22 mois de controle de la population bovine Belge. Ann Med Vet 134: 175–178Google Scholar