Serological and antigenical findings indicating pestivirus in man

  • M. Giangaspero
  • G. Vacirca
  • M. Buettner
  • G. Wolf
  • E. Vanopdenbosch
  • G. Muyldermans
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 7)


An epidemiological survey for pestivirus was undertaken in Zambia and Europe, in view of the recent serological findings obtained by previous studies in Europe with humans. Collected sera were tested for anti-bovine viral diarrhea virus (BVDV) specific antibodies by IIF and Western Blotting. Of those individuals tested (n = 1272), 15.3% showed a seropositive reaction to the BVDV. Anti-BVDV antibody prevalence in immuno-depressed patients (e.g. HIV positive) was investigated. A higher prevalence was revealed in HIV patients suffering from chronic diarrhoea and in those having developed AIDS Related Complex (ARC). Out of 212 persons tested for pestivirus isolation, a non cytopathic virus strain was detected in 2 buffy coat samples using IIF with a specific anti-BVDV serum. The isolation could be repeated three times during 31 days in one person. The virus was identified as a pestivirus with radioimmuno-precipitation assays and IIF-flow cytometry. A doublet of 120 kD was identified only in cell lysates, indicating a non-structural protein. In order to rule out cross reactivity 30 sera from Hepatitis C seropositive patients were tested against the isolate by IIF-flow cytometry. No antigen-specific binding could be observed. These findings indicated the occurrence of a pestivirus in man and might suggest a relationship with a pestivirus of animal origin.


Human Immunodeficiency Virus Human Immunodeficiency Virus Infection West Nile Virus Bovine Viral Diarrhoea Virus Human Immunodeficiency Virus Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. Giangaspero
    • 1
    • 5
  • G. Vacirca
    • 1
  • M. Buettner
    • 2
  • G. Wolf
    • 2
  • E. Vanopdenbosch
    • 3
  • G. Muyldermans
    • 4
  1. 1.Institute of Special Pathology and Veterinary Medical Clinic, Faculty of Veterinary MedicineUniversity of MilanMilanItaly
  2. 2.Institute of Medical Microbiology, Faculty of Veterinary MedicineLudwig Maximilians UniversityMunichFederal Republic of Germany
  3. 3.Department of Bovine VirologyThe National Institute for Veterinary ResearchBrusselsBelgium
  4. 4.Institute of Molecular BiologyFree University of BrusselsSint-Genesius-RodeBelgium
  5. 5.Institute of Special Pathology and Veterinary Medical Clinic, Faculty of Veterinary MedicineUniversity of MilanMilanItaly

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