Serological and antigenical findings indicating pestivirus in man

  • M. Giangaspero
  • G. Vacirca
  • M. Buettner
  • G. Wolf
  • E. Vanopdenbosch
  • G. Muyldermans
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 7)


An epidemiological survey for pestivirus was undertaken in Zambia and Europe, in view of the recent serological findings obtained by previous studies in Europe with humans. Collected sera were tested for anti-bovine viral diarrhea virus (BVDV) specific antibodies by IIF and Western Blotting. Of those individuals tested (n = 1272), 15.3% showed a seropositive reaction to the BVDV. Anti-BVDV antibody prevalence in immuno-depressed patients (e.g. HIV positive) was investigated. A higher prevalence was revealed in HIV patients suffering from chronic diarrhoea and in those having developed AIDS Related Complex (ARC). Out of 212 persons tested for pestivirus isolation, a non cytopathic virus strain was detected in 2 buffy coat samples using IIF with a specific anti-BVDV serum. The isolation could be repeated three times during 31 days in one person. The virus was identified as a pestivirus with radioimmuno-precipitation assays and IIF-flow cytometry. A doublet of 120 kD was identified only in cell lysates, indicating a non-structural protein. In order to rule out cross reactivity 30 sera from Hepatitis C seropositive patients were tested against the isolate by IIF-flow cytometry. No antigen-specific binding could be observed. These findings indicated the occurrence of a pestivirus in man and might suggest a relationship with a pestivirus of animal origin.


Human Immunodeficiency Virus Human Immunodeficiency Virus Infection West Nile Virus Bovine Viral Diarrhoea Virus Human Immunodeficiency Virus Patient 


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  1. 1.
    Bolin SR, McClurkin AW, Coria MF (1985) Frequency of persistent BVDV infection in selected cattle herds. Am J Vet Res 46: 2378–2385Google Scholar
  2. 2.
    Caij A, Muyldermans G, De Smet A, Hamers R, Koenen E (1993) Production and characterization of monoclonal antibodies against hog cholera virus (Alfort 187 strain). Arch Virol 131: 185–192PubMedCrossRefGoogle Scholar
  3. 3.
    Castrucci G (1978) Togaviridae. In: Infezione da virus degli animali domestici. Edagricolae, Milan 3: 114–201Google Scholar
  4. 4.
    Corapi WV, Donis R, Dubovi EJ (1988) Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhoea virus infections. J Virol 62: 2823–2827PubMedGoogle Scholar
  5. 5.
    Franki RIB, Fauquet CM, Knudson DL, Braun F (eds) (1991) Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York (Arch Virol [Suppl] 2 )Google Scholar
  6. 6.
    Giangaspero M (1986) Infezione da virus BVD/Recettivit umana al BVD. Thesis of Doctorat. University of Milan, ItalyGoogle Scholar
  7. 7.
    Giangaspero M (1989) La Diarrhee Virale des Bovins — BVD. Detection d’anticorps specifiques chez l’homme. Thesis of MSc 8 IMT Antwerp BelgiumGoogle Scholar
  8. 8.
    Gianpaspero M, Blondeel H, Bear J, Alders R, Morgan D (1991) Epidemiological survey on Bovine Viral Diarrhoea virus in Zambian cattle. Zimbabwe Vet J 22: 57–63Google Scholar
  9. 9.
    Giangaspero M, Wellemans G, Vanopdenbosch E, Belloli A, Verhulst A (1988) Bovine viral diarrhoea. Lancet ii: 110CrossRefGoogle Scholar
  10. 10.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227: 680–686Google Scholar
  11. 11.
    Muyldermans G, Caij A, De Smet A, Koenen F, Hamers R (1993) Characterization of structural and non-structural proteins of Hog Cholera virus by means of monoclonal antibodies. Arch Virol 131: 405–417PubMedCrossRefGoogle Scholar
  12. 12.
    Potgieter LND, McCracken MD, Hopkins JM (1984) Effect of BVDV infection on the distribution of infectious bovine rhinotracheitis virus in cows. Am J Vet Res 45: 687PubMedGoogle Scholar
  13. 13.
    Qvist P, Houe H, Aasted B, Meyling A (1991) Comparison of flow cytometry and virus isolation in cell culture for identification of cattle persistently infected with bovine viral diarrhea virus. J Clin Microbiol 29: 660–661PubMedGoogle Scholar
  14. 14.
    Reggiardo C, Kaeberle ML (1981) Detection of bacteraemia in cattle inoculated with BVDV. Am J Vet Res 42: 218PubMedGoogle Scholar
  15. 15.
    Stoeber M (1982) Streptotrichose der Haut (Dermatophytose) bei Mastbullen mit BVD. Tieraerztl Umschau 37: 629–630Google Scholar
  16. 16.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76: 4350–4354PubMedCrossRefGoogle Scholar
  17. 17.
    Vacirca G, Giangaspero M, Belloli A, Bertoli G, Citrino A (1988) Ricerca di Anticorpi anti-BVD in siero di sangue umano. Meeting 8: 37–39Google Scholar
  18. 18.
    Wellemans G, Leunen J (1973) La rhinotracheite infectieuse des bovins ( IBR) et sa serologie. Ann Med Vet 117: 507–511Google Scholar
  19. 19.
    Wellemans G, Vanopdenbosch E (1990) Depistage des bovins BVD-immunotolerants (BVD-IT) a partir des lymphocytes: un apercu de 22 mois de controle de la population bovine Belge. Ann Med Vet 134: 175–178Google Scholar
  20. 20.
    Wilks CR, Abraham G, Blackmore DK (1989) Bovine pestivirus and human infection. Lancet i: 107CrossRefGoogle Scholar
  21. 21.
    Yolken R, Leister F, Almeido-Hill J, Dubovi E, Reid R, Santosham M (1989) Infantile gastroenteritis associated with excretion of pestivirus antigens. Lancet i: 517–519CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. Giangaspero
    • 1
    • 5
  • G. Vacirca
    • 1
  • M. Buettner
    • 2
  • G. Wolf
    • 2
  • E. Vanopdenbosch
    • 3
  • G. Muyldermans
    • 4
  1. 1.Institute of Special Pathology and Veterinary Medical Clinic, Faculty of Veterinary MedicineUniversity of MilanMilanItaly
  2. 2.Institute of Medical Microbiology, Faculty of Veterinary MedicineLudwig Maximilians UniversityMunichFederal Republic of Germany
  3. 3.Department of Bovine VirologyThe National Institute for Veterinary ResearchBrusselsBelgium
  4. 4.Institute of Molecular BiologyFree University of BrusselsSint-Genesius-RodeBelgium
  5. 5.Institute of Special Pathology and Veterinary Medical Clinic, Faculty of Veterinary MedicineUniversity of MilanMilanItaly

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