Summary
Molecular characterization has become an important tool for the analysis of viruses including their classification. The manuscript focuses on the molecular analysis of two members of the genus pestivirus (hog cholera virus, HCV and bovine viral diarrhea virus, BVDV) and of the recently discovered porcine reproductive and respiratory syndrome virus (PRRSV). The first protein encoded within the single large pestivirus ORF is a nonstructural protein with autoproteolytic activity. The cleavage site between the protease and the capsid protein p14 has been predicted previously, but recent experimental data indicate that processing occurs at a different site. The capsid protein is followed by a putative internal signal sequence and three glycoproteins which are part of the virion envelope. According to a new proposal for the nomenclature of the structural proteins of pestiviruses they are termed C, E0, El and E2. The genomes of BVDV pairs isolated from animals which came down with mucosal disease were analyzed. The genomes from cytopathogenic (cp) BVD viruses may contain insertions highly homologous to cellular sequences. In addition, cp BVDV may differ from its non cytopathogenic (noncp) counterpart by mere rearrangement of viral sequences. The disease PRRS, which emerged a few years ago, is caused by a single strand RNA virus; the viral genome is of positive polarity and has a size of 15 kb. Data concerning morphology, morphogenesis and virion composition suggested already that PRRSV belongs to a group of so-called arteriviruses which comprises equine arteritis virus (EAV), lactate dehydrogenase elevating virus (LDV) and simian hemorrhagic fever virus (SHFV). This conclusion has now been confirmed by analysis of genome organization, gene expression strategy and by comparison of deduced protein sequences.
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Keywords
- Bovine Viral Diarrhea Virus
- Mucosal Disease
- Equine Arteritis Virus
- Bovine Viral Diarrhea Virus Infection
- Autoproteolytic Activity
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Baker JC (1987) Bovine viral diarrhea virus: A review. J Am Vet Med Assoc 190: 1449–1458
Benfield DA, Nelson E, Collins JE, Harris L, Goyal SM, Robison D, Christianson WT, Morrison RB, Gorcyca D, Chladek D (1992) Characterization of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332). J Vet Diagn Invest 4: 127–133
Bolin SR, McClurkin AW, Cutlip RC, Coria MF (1985) Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. Am J Vet Res 46: 573–576
Brownlie J, Clarke MC, Howard CJ (1984) Experimental production of fatal mucosal disease in cattle. Vet Rec 114: 535–536
Collett MS, Wiskerchen MA, Welniak E, Belzer SK (1991) Bovine viral diarrhea virus genomic organization. In: Liess B, Moennig V, Pohlenz J, Trautwein G (eds) Ruminant pestirus infections. Springer, Wien New York, pp 19–27 (Arch Virol [Suppl] 3 )
Collett MS, Moennig V, Horzinek MC (1989) Recent advances in pestivirus research. J Gen Virol 70: 253–266
Collett MS, Larson R, Belzer SK, Retzel E (1988) Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165: 200–208
Collett MS, Larson R, Gold C, Strinck D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199
Conzelmann K-K, Visser N, Van Woensel P, Thiel H-J (1993) Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology 193: 329–339
Corapi WV, Donis RO, Dubovi EJ (1988) Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. J Virol 62: 2823–2827
Den Boon JA, Snijder EJ, Chirnside ED, De Vries AAF, Horzinek MC, Spaan WJM (1991) Equine arteritis virus is not a togavirus but belongs to the coronavirus superfamily. J Virology 65: 2910–2920
Finley D, Bartel B, Varshaysky A (1989) The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis. Nature 338: 394–401
Finley D, Özkaynak E, Varshaysky A (1987) The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation and other stresses. Cell 48: 1035–1046
Godeny EK, Speicher DW, Brinton MA (1990) Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein ( Vpl) gene. Virology 177: 768–771
Meyers G, Rümenapf T, Thiel H-J (1989a) Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171: 555–567
Meyers G, Rümenapf T, Thiel H-J (1990) Insertion of ubiquitin-coding sequence identified in the RNA genome of a Togavirus. In: Brinton MA, Heinz FX (eds) New aspects of positive strand RNA viruses. American Society for Microbiology, Washington, D.C., pp 25–29
Meyers G, Tautz N, Dubovi EJ, Thiel H-J (1991) Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences. Virology 180: 602–616
Meyers G, Tautz N, Stark R, Brownlie J, Dubovi EJ, Collett MS, Thiel H-J (1992) Rearrangement of viral sequences in cytopathogenic pestiviruses. Virology 191: 368–386
Meyers G, Rümenapf T, Thiel H-J (1989b) Ubiquitin in a togavirus. Nature 341: 491
Özkaynak E, Finley D, Solomonm MS, Varshaysky A (1987) The yeast ubiquitin genes: A family of natural gene fusions. EMBO J 6: 1429–1439
Plagemann PGW, Moennig V (1992) Lactate dehydrogenase-elevating virus, equine arteritis virus and simian hemorrhagic fever virus. Adv Virus Res 41: 99–192
Pocock DH, Howard CJ, Clarke MC, Brownlie J (1987) Variation in the intracellular polypeptide profiles from different isolates of bovine viral diarrhea virus. Arch Virol 94: 43–53
Qi F, Ridpath JF, Lewis T, Bolin SR, Berry ES (1992) Analysis of the bovine viral diarrhea virus genome for possible cellular insertions. Virology 189: 285–292
Rechsteiner M (1987) Ubiquitin-mediated pathways for intracellular proteolysis. Annu Rev Cell Bio1 3: 1–30
H.-J. Thiel et al.: Molecular characterization of positive-strand RNA viruses
Redmann KL, Rechsteiner M (1989) Identification of the long ubiquitin extension as ribosomal protein S27a. Nature 338: 438–440
Renard A, Dino D, Martial J (1987) Vaccines and diagnostics derived from bovine diarrhea virus. European Patent Application number 86870095.6. Publication number 0208672, 14 January 1987
Stark R, Rümenapf T, Meyers G, Thiel H-J (1990) Genomic localization of hog cholera virus glycoproteins. Virology 174: 286–289
Thiel H-J, Stark R, Weiland E, Rümenapf T, Meyers G (1991) Hog cholera virus: molecular composition of virions from a pestivirus. J Virol 65: 4705–4712
Weiland E, Stark R, Haas B, Rümenapf T, Meyers G, Thiel H-J (1990) Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer. J Virol 64: 3563–3569
Weiland E, Ahl R, Stark R, Weiland F, Thiel H-J (1992) A second envelope glycoprotein mediates neutralization of a pestivirus, hog cholera virus. J Virol 66: 3677–3682
Westaway EG, Brinton MA, Gaidamovich SYA, Horzinek MC, Igarashi A, Kääriäinen L, Lvov DK, Porterfield JS, Russel PK, Trent DW (1985) Togaviridae. Intervirology 24: 125–139
Wensvoort G, Terpstra C, Pol JMA, Wagenaar F (1991) Lelystad virus, the cause of porcine epidemic abortion and respiratory syndrome (mystery swine disease). In: Proc Porcine Reprod Respir Syndrome Semin, November 4–5, Brussels, Belgium, pp 27–35. European Commission VI/BII.2
Wiskerchen MA, Belzer SK, Collett MS (1991) Pestivirus gene expression: The first protein of the bovine viral diarrhea virus large open reading frame, p20, possesses proteolytic activity. J Virol 65: 4508–4514
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© 1993 Springer-Verlag
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Thiel, HJ. et al. (1993). Molecular characterization of positive-strand RNA viruses: pestiviruses and the porcine reproductive and respiratory syndrome virus (PRRSV). In: Kaaden, OR., Eichhorn, W., Czerny, CP. (eds) Unconventional Agents and Unclassified Viruses. Archives of Virology, vol 7. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9300-6_4
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DOI: https://doi.org/10.1007/978-3-7091-9300-6_4
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