Establishment of cell lines from bovine brain

Brief Report
  • A. Uysal
  • O.-R. Kaaden
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 7)


As shown by immunocytochemistry, 16 cell lines of neuronal, oligodendroglial or neuronoglial origin have been established from bovine fetal brain by immortalization with SV40 virus and cloning in soft agar. The cell lines were characterized according to their cell surface markers using mono- and polyclonal antibodies.


Soft Agar Bovine Spongiform Encephalopathy Bovine Brain Brain Cell Culture Scrapie Agent 
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  1. 1.
    Asher DM, Yanagiha RT, Rogers NG, Gibbs CJ, Gajdusek DC (1979) Studies of the viruses of spongiform encephalopathies. In: Prusiner SB, Hadlow WJ (eds) Slow transmissible diseases of the nervous system. Academic Press, New York, pp 235–242Google Scholar
  2. 2.
    Buening GM, Gustafson GP (1971) Growth characteristics of scrapie agent infected mouse brain cell cultures. Am J Vet Res 32: 953–963PubMedGoogle Scholar
  3. 3.
    Butler DA, Scott MRD, Bockman JM, Borchelt DR, Taraboulos A, Hsiao KK, Kingsbury DT, Prusiner SB (1988) Scrapie-infected murine neuroblastoma cells produce protease-resistent prion proteins. J Virol 62: 1558–1564PubMedGoogle Scholar
  4. 4.
    Caspary EA, Bell TM (1971) Growth potential of scrapie mouse brain in vitro. Nature 229: 269–271PubMedCrossRefGoogle Scholar
  5. 5.
    Cattaneo E, McKay R (1990) Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature 347: 762–765PubMedCrossRefGoogle Scholar
  6. 6.
    Cherednichenko YN, Mikhailova GR, Rajcani J, Zhdanov VM (1985) In vitro studies with the scrapie agent. Acta Virol 29: 285–293PubMedGoogle Scholar
  7. 7.
    Clarke MC, Haig DA (1970) Evidence for the multiplication of scrapie agent in cell culture. Nature 225: 100–101PubMedCrossRefGoogle Scholar
  8. 8.
    Clarke MC, Millson GC (1976) Infection of a cell line of mouse L fibroblasts with scrapie agent. Nature 261: 144–145PubMedCrossRefGoogle Scholar
  9. 9.
    Engele J, Churchill Bohn M (1991) The neurotrophic effects of fibroblast growth factors on dopaminergic neurons in vitro are mediated by mesencephalic glia. J Neurosci 11: 3070–3078PubMedGoogle Scholar
  10. 10.
    Field EJ, Windsor GD (1965) Culture characteristics of scrapie mouse brain. Res Vet Sci 6: 130–132PubMedGoogle Scholar
  11. 11.
    Gustafson DP, Kanitz CL (1965) Evidence of the presence of scrapie in cell culture of brain. In: Gajdusek DC, Gibbs CJ, Alpers M (eds) Slow, latent and temperate A. Uysal and O.-R. Kaaden: Establishment of cell lines from bovine brain virus infection. National Institute of Neurological Diseases and Blindness, Monograph No. 2. Bethesda, pp 221–236Google Scholar
  12. 12.
    Haig DA, Pattison IH (1967) In vitro growth of pieces of brain from scrapieaffected mice. J Pathol Bact 93: 724–727CrossRefGoogle Scholar
  13. 13.
    Hartikka J, Hefti F (1988) Development of septal cholinergic neurons in culture: plating density and glial cells modulate effects of NGF on survival, fiber growth, and expression of transmitter-specific enzymes. J Neurosci 8: 2967–2985PubMedGoogle Scholar
  14. 14.
    Hewicker M, Trautwein G (1991) Identifizierung von Zellen des Zentralnervensystems mittels zellspezifischer Marker. Tierärztl Prax 19: 241–246PubMedGoogle Scholar
  15. 15.
    Markovits P, Dormont D, Delpeck B, Court L, Lararjet R (1981) Essais de propagation in vitro de l’agent scrapie dans des cellules nerveuses de souris. Comptes rendus hebdomadaires des seances de l’Academie des sciences 293: 413–417Google Scholar
  16. 16.
    Mizuguchi M, Yamada M, Kim SU, Rhee SG (1991) Phospholipase C isozymes in neurons and glial cells in culture: an immunocytochemical and immunochemical study. Brain Res 548: 35–40PubMedCrossRefGoogle Scholar
  17. 17.
    Osborn M, Weber K (1983) Tumor diagnosis by intermediate filament typing: A novel tool for surgical pathology. Lab Invest 48: 372–394PubMedGoogle Scholar
  18. 18.
    Pixley SK, Pun RK (1990) Cultured rat olfactory neurons are excitable and respond to odors. Dev Brain Res 53: 125–130CrossRefGoogle Scholar
  19. 19.
    Race RE, Fadness LH, Chesebro B (1987) Characterization of scrapie infection in mouse neuroblastoma cells. J Gen Virol 68: 1391–1399PubMedCrossRefGoogle Scholar
  20. 20.
    Roikhel VM, Fokina GI, Lisak VM, Kondakova LI, Korolev MB, Pogodina VV (1987) Persistence of the scrapie agent in glial cells from rat Gasserian ganglion. Acta Virol Praha 31: 36–42PubMedGoogle Scholar
  21. 21.
    Rubenstein R, Carp R, Callahan SM (1984) In vitro replication of scrapie agent in a neuronal model: infection of PC 12 cells. J Gen Virol 65: 2191–2198PubMedCrossRefGoogle Scholar
  22. 22.
    Taraboulos A, Serban D, Prusiner SB (1990) Scrapie prion proteins accumulate in the cytoplasm of persistently infected cultured cells. J Cell Biol 110: 2117–2132PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • A. Uysal
    • 1
    • 2
  • O.-R. Kaaden
    • 1
    • 2
  1. 1.Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin,Tierärztliche FakultätLudwig-Maximilians-Universität MünchenMunichFederal Republic of Germany
  2. 2.Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary FacultyLudwig-Maximilians-University MunichMunichFederal Republic of Germany

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