Distribution of cytopathogenic and noncytopathogenic bovine virus diarrhea virus in tissues from a calf with experimentally induced mucosal disease using antigenic and genetic markers

Brief Report
  • I. Greiser-Wilke
  • E. Liebler
  • L. Haas
  • B. Liess
  • J. Pohlenz
  • V. Moennig
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 7)


A comparative analysis of the distribution of cytopathogenic (cp) and noncytopathogenic (ncp) bovine virus diarrhea disease (BVD) virus in tissues from a calf with experimentally induced mucosal disease was performed using immunohistology and polymerase chain reaction after reverse transcription (RT-PCR) of viral RNA. For immunohistology, an antigenic marker on the superinfecting cp BVD virus defined by a monoclonal antibody (mab) was used, and overall presence of antigen was assessed with a pestivirus specific mab. The primers selected for RT-PCR detected the genomic insertion in the p125 region of the superinfecting cp BVD virus. Both methods gave consistent results.


Viral Antigen Bovine Viral Diarrhea Virus Lymphoid Follicle Bovine Viral Diarrhea Diarrhea Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bolin SR, McClurkin AW, Cutlip RC, Coria MF (1985) Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. Am J Vet Res 46: 573–576PubMedGoogle Scholar
  2. 2.
    Brownlie J, Clarke MC, Howard CJ (1984) Experimental production of fatal mucosal disease in cattle. Vet Rec 114: 535–536PubMedCrossRefGoogle Scholar
  3. 3.
    Collett MS, Larson R, Gold C, Strick D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199CrossRefGoogle Scholar
  4. 4.
    Donis RO, Dubovi EJ (1987) Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. Virology 158: 168–173PubMedCrossRefGoogle Scholar
  5. 5.
    Greiser-Wilke I, Haas L, Dittmar KE, Liess B, Moennig V (1993) RNA insertions and gene duplications in the nonstructural protein p125 region of pestivirus strains and isolates in vitro and in vivo. Virology 193: 977–980PubMedCrossRefGoogle Scholar
  6. 6.
    Hooft van Iddekinge BJL, van Wamel JLB, van Gennip HGP, Moormann RJM (1992) Application of the polymerase chain reaction to the detection of bovine viral diarrhea virus infections in cattle. Vet Microbiol 30: 31–34Google Scholar
  7. 7.
    Liebler EM, Waschbusch J, Pohlenz JF, Moennig V, Liess B (1991) Distribution of antigen of noncytopathogenic and cytopathogenic bovine virus diarrhea virus biotypes in the intestinal tract of calves following experimental production of mucosal disease. In: Liess B, Moennig V, Pohlenz J, Trautwein G (eds) Ruminant pestivirus infections. Springer, Wien, New York, pp 109–124 (Arch Virol [Suppl] 3 )Google Scholar
  8. 8.
    McClurkin AW, Bolin SR, Coria MF (1985) Isolation of cytopathic and noncytopathic bovine viral diarrhea virus from the spleen of cattle acutely and chronically affected with bovine viral diarrhea. J Am Vet Med Assoc 186: 568–569PubMedGoogle Scholar
  9. 9.
    Meyers G, Rumenapf T, Thiel H-J (1989) Ubiquitin in a togavirus. Nature 341: 491PubMedCrossRefGoogle Scholar
  10. 10.
    Meyers G, Tautz N, Dubovi EJ, Thiel H-J (1991) Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences. Virology 180: 602–616PubMedCrossRefGoogle Scholar
  11. 11.
    Meyers G, Tautz N, Stark R, Brownlie J, Dubovi EJ, Collett MS, Thiel H-J (1992) Rearrangement of viral sequences in cytopathogenic pestiviruses. Virology 191: 368–386PubMedCrossRefGoogle Scholar
  12. 12.
    Moennig V, Frey H-R, Liebler E, Pohlenz J, Liess B (1990) Reproduction of mucosal disease with cytopathogenic bovine viral diarrhoea virus selected in vitro. Vet Rec 127: 200–203PubMedGoogle Scholar
  13. 13.
    Peters W, Greiser-Wilke I, Moennig V, Liess B (1986) Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet Microbiol 12: 195–200PubMedCrossRefGoogle Scholar
  14. 14.
    Pocock DH, Howard CJ, Clarke MC, Brownlie J (1987) Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. Arch Virol 94: 43–53PubMedCrossRefGoogle Scholar
  15. 15.
    Purchio AF, Larson R, Collett MS (1984) Characterization of bovine viral diarrhea virus proteins. J Virol 50: 666–669PubMedGoogle Scholar
  16. 16.
    Qi F, Ridpath JF, Lewis T, Bolin SR, Berry ES (1992) Analysis of the bovine viral diarrhea virus genome for possible cellular insertions. Virology 189: 285–292PubMedCrossRefGoogle Scholar
  17. 17.
    Wengler G (1991) Family Flaviviridae. In: Francki RIB, Fauquet CM, Knudson DL, Brown F (eds) Classification and nomenclature of viruses. Springer, Wien New York, pp 223–233 (Arch Virol [Suppl] 2 )Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • I. Greiser-Wilke
    • 1
  • E. Liebler
    • 2
  • L. Haas
    • 1
  • B. Liess
    • 1
  • J. Pohlenz
    • 2
  • V. Moennig
    • 1
    • 3
  1. 1.Institute of VirologyHannover Veterinary SchoolHannoverFederal Republic of Germany
  2. 2.Institute of PathologyHannover Veterinary SchoolHannoverFederal Republic of Germany
  3. 3.Institute of VirologyHannover Veterinary SchoolHannoverFederal Republic of Germany

Personalised recommendations