Distribution of cytopathogenic and noncytopathogenic bovine virus diarrhea virus in tissues from a calf with experimentally induced mucosal disease using antigenic and genetic markers

Brief Report
  • I. Greiser-Wilke
  • E. Liebler
  • L. Haas
  • B. Liess
  • J. Pohlenz
  • V. Moennig
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 7)


A comparative analysis of the distribution of cytopathogenic (cp) and noncytopathogenic (ncp) bovine virus diarrhea disease (BVD) virus in tissues from a calf with experimentally induced mucosal disease was performed using immunohistology and polymerase chain reaction after reverse transcription (RT-PCR) of viral RNA. For immunohistology, an antigenic marker on the superinfecting cp BVD virus defined by a monoclonal antibody (mab) was used, and overall presence of antigen was assessed with a pestivirus specific mab. The primers selected for RT-PCR detected the genomic insertion in the p125 region of the superinfecting cp BVD virus. Both methods gave consistent results.


Viral Antigen Bovine Viral Diarrhea Virus Lymphoid Follicle Bovine Viral Diarrhea Diarrhea Virus 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • I. Greiser-Wilke
    • 1
  • E. Liebler
    • 2
  • L. Haas
    • 1
  • B. Liess
    • 1
  • J. Pohlenz
    • 2
  • V. Moennig
    • 1
    • 3
  1. 1.Institute of VirologyHannover Veterinary SchoolHannoverFederal Republic of Germany
  2. 2.Institute of PathologyHannover Veterinary SchoolHannoverFederal Republic of Germany
  3. 3.Institute of VirologyHannover Veterinary SchoolHannoverFederal Republic of Germany

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