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Measles virus antigenic variations and the role of individual antigens in immunization

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Measles and Poliomyelitis

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Summary

Measles remains one of the major childhood diseases. Advances in genetic manipulation make possible new approaches to vaccination. However, a number of problems need to be studied before such a vaccine could be considered for field trials. (1) Does measles virus exhibit antigenic variation and if so what is its significance in protection. (2) Which antigen (s) should be incorporated in future vaccines. In the present study we compared field isolates and vaccine virus strains using monoclonal antibodies. No antigenic variation was observed on either of the two glycoproteins, the haemagglutinin (HA) and the fusion (F) proteins. In contrast, differences were observed on the nucleoprotein (NP) and one of the epitopes distinguished wild-type and vaccine strains.

To investigate the role of the different measles virus proteins in immunization, the measles virus proteins were expressed using vaccinia virus (VV) as a vector. Immunization with the HA or F recombinants, but not the NP protected mice against a lethal challenge. Protection with the F recombinant depended on the haplotype of the animal, but a low response to this antigen could be increased by the co-expression with the NP protein. Immunization of mice with the VV-recombinants expressing the F, NP or M (matrix) proteins, but not the HA, partially protected animals from a lethal challenge with canine distemper virus. In the case of the NP and M the protection was not mediated by antibodies.

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References

  1. Appel MJG, Shek WR, Sheshberadaran H, Norrby E (1984) Measles virus and inactivated canine distemper virus induce incomplete immunity to canine distemper. Arch Virol 82: 73–82

    Article  PubMed  CAS  Google Scholar 

  2. Barrett T, Shrimpton SB, Russel SEH (1985) Nucleotide sequence of the entire protein encoding region of canine distemper virus polymerase-associated (P) protein mRNA. Virus Res 3: 367–372

    Article  PubMed  CAS  Google Scholar 

  3. Barrett T, Clarke DK, Evans SA, Rima BK (1987) The nucleotide sequence of the gene encoding the F protein of canine distemper virus: a comparison of the deduced amino acid sequence with other paramyxoviruses. Virus Res 8: 373–386

    Article  PubMed  CAS  Google Scholar 

  4. Bellini WJ, Englund G, Rozenblatt S, Arnheiter H, Richardson CD (1985) Measles virus P gene codes for two proteins. J Virol 53: 908–919

    PubMed  CAS  Google Scholar 

  5. Bellini WJ, Englund G, Richardson CD, Rozenblatt S, Lazzarini RA (1986) Matrix genes of measles virus and canine distemper virus: cloning, nucleotide sequences and deduced amino acid sequences. J Virol 58: 408–416

    PubMed  CAS  Google Scholar 

  6. Black FL (1991) Epidemiology of paramyxoviridae. In: Kingsbury DW (ed) The Paramyxoviruses. Plenum Press, New York, pp 509–536

    Google Scholar 

  7. Buckland R ,Gerald C ,Barker R ,Wild TF (1987) Fusion glycoprotein of measles virus: nucleotide sequence of the gene and comparison with other paramyxoviruses. J Gen Virol 68: 1695–1703

    Article  PubMed  CAS  Google Scholar 

  8. Buckland R, Gerald C, Barker R ,Wild TF (1988) Cloning and sequencing of the nucleoprotein gene of measles virus (Hallé strain). Nucleic Acids Res 16: 11821

    Article  PubMed  CAS  Google Scholar 

  9. Buckland R ,Giraudon P, Wild TF (1989) Expression of Measles virus nucleoprotein in Escherichia coli: use of deletion mutants to locate the antigenic sites. J Gen Virol 70: 435–441

    Article  PubMed  CAS  Google Scholar 

  10. Buller RML ,Smith GL, Cremer K ,Notkins AL, Moss B (1985) Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinasenegative phenotype. Nature 317: 813–815

    Article  PubMed  CAS  Google Scholar 

  11. Curran MD, Clarke DK, Rima BK (1991) The nucleotide sequence of the gene encoding the attachment protein H of canine distemper virus. J Gen Virol 72: 443–447

    Article  PubMed  CAS  Google Scholar 

  12. Drillien R. Spehner D, Kirn A, Giraudon P, Buckland R, Wild TF ,Lecocq JP (1988) Protection of mice from fatal measles encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein. Proc Natl Acad Sci USA 85: 1252–1256

    Article  PubMed  CAS  Google Scholar 

  13. Ertl HCJ ,Dietzschold B ,Gore M ,Otuosjr L ,Larson JK ,Wunner WH ,Koprowski H (1989) Induction of rabies virus-specific T-helper cells by synthetic peptides that carry dominant T-helper cell epitopes of the viral ribonucleoprotein. J Virol 63: 2885–2892

    PubMed  CAS  Google Scholar 

  14. Fulginiti VA ,Eller JJ, Downie AW, Kempe CH (1967) Altered reactivity to measles virus. Atypical measles in children previously immunized with inactivated measles virus vaccines. J Am Med Ass 202: 1075–1080

    Article  CAS  Google Scholar 

  15. Gerald C, Buckland R, Barker R, Freeman G, Wild TF (1986) Measles virus haemagglutinin gene: cloning ,complete sequence analysis and expression in COS cells. J Gen Virol 67: 2695–2703

    Article  PubMed  CAS  Google Scholar 

  16. Giraudon P ,Wild TF (1981a) Monoclonal antibodies against measles virus. J Gen Virol 54: 325–332

    Article  PubMed  CAS  Google Scholar 

  17. Giraudon P ,Wild TF (1981b) Differentiation of measles virus strains and a strain of canine distemper virus by monoclonal antibodies. J Gen Virol 57: 179–183

    Article  PubMed  CAS  Google Scholar 

  18. Giraudon P ,Wild TF (1985) Correlation between epitopes on hemagglutinin of measles virus and biological activities: passive protection by monoclonal antibodies is related to their hemagglutination inhibiting activity. Virology 144: 46–58

    Article  PubMed  CAS  Google Scholar 

  19. Giraudon P ,Jacquier MF ,Wild TF (1988) Antigenic analysis of African measles virus field isolates: identification and localisation of one conserved and two variable epi tope sites on the NP protein. Virus Res 18: 137–152

    Article  Google Scholar 

  20. Limo M ,Yilma T (1990) Molecular cloning of the rinderpest virus matrix gene: compara tive sequence analysis with other paramyxoviruses. Virology 175: 323–327

    Article  PubMed  CAS  Google Scholar 

  21. Malvoisin E ,Wild TF (1990) Contribution of measles virus fusion protein in protective immunity: anti-F monoclonal antibodies neutralize virus infectivity and protect mice against challenge. J Virol 64: 5160–5162

    PubMed  CAS  Google Scholar 

  22. Norrby E ,Enders-Ruckle G ,Ter Meulen V (1975) Differences in the appearance of antibodies to structural components of measles virus after immunization with inactivated and live virus. J Inf Dis 132: 262–269

    Article  CAS  Google Scholar 

  23. Norrby E ,Sheshberadaran H ,McCullough KC ,Carpenter WC ,Örvell C (1985) Is rinderpest virus the archevirus of the morbillivirus genus? Intervirology 23: 228–232

    Article  PubMed  CAS  Google Scholar 

  24. Panum PL (1940) Observations made during the epidemic of measles on the Faroe islands in the year 1846. American Publishing Association ,New York

    Google Scholar 

  25. Reiss CS,Evans GA ,Margulies DH ,Seidman JG, Burakoff SJ (1983) Allospecific and virus-specific cytolytic T lymphocytes are restricted to the N or C1 domain of H-2 antigens expressed on L cells after DNA-mediated gene transfer. Proc Natl Acad Sci USA 80: 2709–2712

    Article  PubMed  CAS  Google Scholar 

  26. Rozenblatt S, Eizenberg O,Ben-Levy R,Lavie V,Bellini WJ (1985) Sequence homology within the morbilliviruses. J Virol 53: 684–690

    PubMed  CAS  Google Scholar 

  27. Sheshberadaran H,Norrby E,McCullough KC,Carpenter WC,Örvell C (1986) The antigenic relationship between measles, canine distemper and rinderpest viruses studied with monoclonal antibodies. J Gen Virol 67: 1381–1392

    Article  PubMed  Google Scholar 

  28. Taylor MJ, Godfrey E, Baczko K, ter Meulen V, Wild TF, Rima B (1991) Identification of several different lineages of measles virus. J Gen Virol 72:439–442

    Article  Google Scholar 

  29. Ter Meulen V, Loffler S, Carter MJ, Stephenson JR (1981) Antigenic characterization of measles and SSPE virus haemagglutinin by monoclonal antibodies. J Gen Virol 57:357–364

    Article  PubMed  Google Scholar 

  30. Tite JP, Russell SM, Dougan G, O’Callaghan D, Jones I, Brownlee G, Liew FY (1988) Antiviral immunity induced by recombinant nucleoprotein of influenza A virus. I. Characterization and cross-reactivity of T cell responses. J Immunol 141: 3980–3987

    PubMed  CAS  Google Scholar 

  31. Tsukiyama K, Sugiyama M, Yoshikawa Y, Yamanouchi K (1987) Molecular cloning and sequence analysis of the rinderpest virus mRNA encoding the hemagglutinin protein. Virology 160: 48–54

    Article  PubMed  CAS  Google Scholar 

  32. Tsukiyama K, Yoshikawa Y, Yamanouchi K (1988) Fusion glycoprotein (F) of rinderpest virus: entire nucleotide sequence of the F mRNA, and several features of the F protein. Virology 164:523–530

    Article  PubMed  CAS  Google Scholar 

  33. Van Wyke Coelingh KL, Winter C, Murphy BR (1985) Antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. Virology 143:569–583

    Article  Google Scholar 

  34. Wild TF, Giraudon P, Bernard A, Huppert J (1979) Isolation and characterization of a defective measles virus from a subacute sclerosing panencephalitis patient. J Med Virol 4: 103–114

    Article  PubMed  CAS  Google Scholar 

  35. Wild TF, Malvoisin E, Buckland R (1991) Measles virus: both the haemagglutinin and fusion glycoproteins are required for fusion. J Gen Virol 72: 439–442

    Article  PubMed  CAS  Google Scholar 

  36. Wild TF, Bernard A, Spehner D, Drillien R (1992) Construction of vaccinia virus recombinants expressing several measles virus proteins and analysis of their efficacy in vaccination of mice. J Gen Virol 73: 359–367

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Unité d’ Immunologie et Stratégie Vaccinale, Institut Pasteur, Lyon, France

    T. F. Wild

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  1. T. F. Wild
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Editors and Affiliations

  1. Faculty of Medicine, University of Montreal, Montreal, Canada

    Edouard Kurstak (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization) (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization)

  2. International Comparative Virology Organization, Montreal, Canada

    Edouard Kurstak (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization) (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization)

  3. Medical Research Centre, King Fahad Hospital, Riyadh, Saudi Arabia

    Edouard Kurstak (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization) (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization)

  4. Viral Diseases Panel, World Health Organization, Geneva, Switzerland

    Edouard Kurstak (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization) (President - International Comparative Virology Organization, Member - Viral Diseases Panel, World Health Organization)

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© 1993 Springer-Verlag/Wien

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Wild, T.F. (1993). Measles virus antigenic variations and the role of individual antigens in immunization. In: Kurstak, E. (eds) Measles and Poliomyelitis. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9278-8_13

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  • DOI: https://doi.org/10.1007/978-3-7091-9278-8_13

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82436-8

  • Online ISBN: 978-3-7091-9278-8

  • eBook Packages: Springer Book Archive

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