Measles virus antigenic variations and the role of individual antigens in immunization

  • T. F. Wild


Measles remains one of the major childhood diseases. Advances in genetic manipulation make possible new approaches to vaccination. However, a number of problems need to be studied before such a vaccine could be considered for field trials. (1) Does measles virus exhibit antigenic variation and if so what is its significance in protection. (2) Which antigen (s) should be incorporated in future vaccines. In the present study we compared field isolates and vaccine virus strains using monoclonal antibodies. No antigenic variation was observed on either of the two glycoproteins, the haemagglutinin (HA) and the fusion (F) proteins. In contrast, differences were observed on the nucleoprotein (NP) and one of the epitopes distinguished wild-type and vaccine strains.

To investigate the role of the different measles virus proteins in immunization, the measles virus proteins were expressed using vaccinia virus (VV) as a vector. Immunization with the HA or F recombinants, but not the NP protected mice against a lethal challenge. Protection with the F recombinant depended on the haplotype of the animal, but a low response to this antigen could be increased by the co-expression with the NP protein. Immunization of mice with the VV-recombinants expressing the F, NP or M (matrix) proteins, but not the HA, partially protected animals from a lethal challenge with canine distemper virus. In the case of the NP and M the protection was not mediated by antibodies.


Measle Virus Antigenic Variation Canine Distemper Virus Lethal Challenge Measle Virus Infection 


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Copyright information

© Springer-Verlag/Wien 1993

Authors and Affiliations

  • T. F. Wild
    • 1
  1. 1.Unité d’ Immunologie et Stratégie VaccinaleInstitut PasteurLyonFrance

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