Principles and Fundamentals of Microvascular Corrosion Casting for SEM Studies

  • S. H. Aharinejad
  • A. Lametschwandtner


Microvascular corrosion casts should ideally enable a thorough study to be made of the whole vascular bed of any tissue or organ by the scanning electron microscope. To get the whole blood vascular system cast the necessary casting media have to fulfil a series of criteria. According to several authors [1–9] the ideal casting media should meet the following criteria (slightly modified)
  1. 1)

    Be nontoxic for the investigator and for the system to be cast.

  2. 2)

    Be of sufficient low viscosity and particle size to pass through smallest blood vessels (5 µm).

  3. 3)

    Be physiologically inert in the system to be cast.

  4. 4)

    Polymerize within an adjustable period of time (3–15 minutes).

  5. 5)

    Not shrinkage during polymerization.

  6. 6)

    Enable microdissection leaving surrounding tissue intact.

  7. 7)

    Resist corrosion (maceration) without dramatic damage to cast surface structures.

  8. 8)

    Be visible in the dissecting microscope after corrosion (maceration).

  9. 9)

    Not change spatial configuration during drying processes.

  10. 10)

    Be electron conductive.

  11. 11)

    Resist the electron bombardment during SEM inspection.

  12. 12)

    Replicate all topographical details of cast endothelial luminal surfaces.

  13. 13)

    Indicate the direction of blood flow in the system cast.



Vasoactive Drug Capillary Viscosimeter Replication Quality Resin Viscosity Casting Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag/Wien 1992

Authors and Affiliations

  • S. H. Aharinejad
    • 1
  • A. Lametschwandtner
    • 2
  1. 1.First Department of AnatomyUniversity of ViennaViennaAustria
  2. 2.Department of Experimental ZoologyUniversity of SalzburgSalzburgAustria

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