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Molecular aspects of the neuronal noradrenaline transporter

  • H. Bönisch
  • G. Paulus
  • G. Martiny-Baron
  • B. Lingen
  • D. Coppeneur
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 34)

Summary

The neuronal noradrenaline transporter was partially purified by means of low and high pressure liquid chromatography using anion exchange, gel filtration and lectin affinity columns. A protein characterized by a molecular weight of 50–53 kilodalton was enriched; it may represent the transporter or a component of it. In addition, a RNA fraction characterized by a mean size of 2 kilobases was isolated from PC12 rat phaeochromocytoma cells and from bovine adrenal medulla; this RNA fraction caused expression of the noradrenaline transporter after microinjection into Xenopus laevis oocytes.

Keywords

PC12 Cell High Pressure Liquid Chromatography Xenopus Laevis Oocyte Lectin Affinity Lauroyl Sarcosine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • H. Bönisch
    • 1
  • G. Paulus
    • 1
  • G. Martiny-Baron
    • 1
  • B. Lingen
    • 1
  • D. Coppeneur
    • 1
  1. 1.Department of Pharmacology and ToxicologyUniversity of BonnBonn 1Federal Republic of Germany

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