Stimulation of noradrenaline release in the cerebral cortex via presynaptic N-methyl-D-aspartate (NMDA) receptors and their pharmacological characterization
In rat brain cortex synaptosomes superfused with Mg2+-free solution containing glycine, [3H]noradrenaline (3H-NA) release evoked by NMD A was abolished by omission of glycine or Ca2+ and inhibited by Mg2+, the competitive and noncompetitive NMDA receptor antagonists CGP 37849 and dizocilpine (MK-801), respectively, as well as by ethanol, but was not affected by tetrodotoxin. The 3H-NA release evoked by L-glutamate was also competitively inhibited by CGP 37849. In conclusion, presynaptic NMDA receptors mediate stimulation of NA release. Ethanol probably acts at this receptor system.
KeywordsNMDA Receptor Noradrenaline Release Kainate Receptor Excitatory Amino Acid Receptor Cortical Synaptosome
Unable to display preview. Download preview PDF.
- Fagg GE, Pozza MF, Olpe HR, Brugger F, Baumann P, Bittiger H, Schmutz M, Angst C, Brundish D, Allgeier H, Heckendorn R, Dingwall JG (1989) CGP 37849: characterization of a novel and potent competitive N-methyl-D-aspartate ( NMDA) receptor antagonist. Br J Pharmacol 97: 582PGoogle Scholar
- Fink K, Göthert M, Molderings G, Schlicker E (1989) N-Methyl-D-aspartate (NMDA) receptor-mediated stimulation of noradrenaline release, but not of other neurotransmitters, in the rat brain cortex: receptor location, characterization and desensitization. Naunyn-Schmiedebergs Arch Pharmacol 339: 514–521PubMedCrossRefGoogle Scholar
- Furchgott RF (1972) The classification of adrenoceptors (adrenergic receptors). An evaluation from the standpoint of receptor theory. In: Blaschko H, Muscholl E (eds) Handbook of experimental pharmacology, vol 33. Springer, Berlin Heidelberg New York, pp 283–335Google Scholar
- Göthert M (1979) Modification of catecholamine release by anaesthetics and alcohols. In: Paton DM (ed) The release of catecholamines from adrenergic neurons. Pergamon Press, Oxford New York, pp 241–261Google Scholar
- Göthert M, Fink K (1989) Inhibition of N-methyl-D-aspartate ( NMDA)- and L- glutamate-induced noradrenaline and acetylcholine release in the rat brain by ethanol. Naunyn-Schmiedebergs Arch Pharmacol 340: 516–521Google Scholar