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Genes of human catecholamine-synthesizing enzymes

  • Conference paper
Parkinson’s Disease. From Clinical Aspects to Molecular Basis

Part of the book series: Key Topics in Brain Research ((KEYTOPICS))

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Summary

We cloned full-length cDNAs and genomic DNAs of human catocholamine-synthesizing enzymes, i.e., tyrosine hydroxylase (TH), aromatic L-amino acid decarboxylase (AADC), dopamine β-hydroxylase (DBH), and phenythanolamine N-methyltransferase (PNMT), and determined the nucleotide sequences and the deduced amino acid sequences. Multiple mRNAs of human TH, human DBH, and human PNMT were discovered by cDNA cloning. Four types of human TH mRNAs are produced by althernative splicing mechanisms from a single gene. The multiple forms of human TH may give additional regulation to the human enzyme. We have succeeded in expressing human TH gene in transgnic mice. The 5′-flanking regions of the genes of human TH, DBH and PNMT contain possible transcription regulatory elements such as cyclic AMP responsive element (CRE) (TH, DBH, PNMT), glucocordicoid responsive element (GRE) (DBH, PNMT), and Sp1 (TH, PNMT) binding site.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan

    Prof. Dr. T. Nagatsu, N. Kaneda, K. Kobayashi, H. Ichinose, T. Sasaoka, A. Ishii & C. Sumi

  2. Radioisotope Center Medical Division, Nagoya University School of Medicine, Nagoya, Japan

    K. Kiuchi

  3. Institute for Comprehensive Medical Science, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan

    K. Fujita & Y. Kurosawa

Authors
  1. Prof. Dr. T. Nagatsu
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  2. N. Kaneda
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  3. K. Kobayashi
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  4. H. Ichinose
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  5. T. Sasaoka
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  6. A. Ishii
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  7. C. Sumi
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  8. K. Kiuchi
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  9. K. Fujita
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  10. Y. Kurosawa
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Editor information

Editors and Affiliations

  1. Department of Neurochemistry, Fujita Health University, Aichi, Japan

    Toshiharu Nagatsu

  2. Neurological Clinic, Tokyo, Japan

    Hirotaro Narabayashi

  3. Department of Neurology, Jichi Medical School, Tochigi-ken, Japan

    Mitsuo Yoshida

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© 1991 Springer-Verlag/Wien

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Nagatsu, T. et al. (1991). Genes of human catecholamine-synthesizing enzymes. In: Nagatsu, T., Narabayashi, H., Yoshida, M. (eds) Parkinson’s Disease. From Clinical Aspects to Molecular Basis. Key Topics in Brain Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9146-0_3

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  • DOI: https://doi.org/10.1007/978-3-7091-9146-0_3

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82272-2

  • Online ISBN: 978-3-7091-9146-0

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