Human blood platelet as research tool in neuropsychopharmacology

  • P. Bongioanni
  • F. Dadone
  • M. Donato
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 33)


The use of blood platelets as a nerve terminal model for serotonin is well documented. However, it is clear that the use of platelets as a model can be justified only for those parameters where it may be shown that blood platelets and neural cells share almost identical features. The excellent similarity between the serotonin transport mechanisms in platelets and in nerve terminals, and the existence of various receptors for biogenic amines, peptides and substances with neuronal activity on platelet membrane offer a really unique opportunity to utilize blood platelets as a system for drug evaluation. In our work platelet benzodiazepine binding sites and their modulation by different benzodiazepines in normals and in demented patients are examined.


Human Platelet Blood Platelet Platelet Membrane Human Blood Platelet Diffuse Neuroendocrine System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Asscher Y, Avnir D, Rotman A, Agranat I (1981) Active conformation of polycyclic antidepressants. J Pharmacol Sci 71: 122–124Google Scholar
  2. Bongioanni P (1987) Platelets as a model in neurological studies: biochemical and clinical features. Arch Psicol Neurol Psichiat 48: 169–192Google Scholar
  3. Bongioanni P (1988) Platelets as a model in psychiatric studies. NPS 8: 582–618.Google Scholar
  4. Bongioanni P (1989) Platelets and schizophrenia: platelet monoamine oxidase activity in schizophrenics. Riv Psichiat 24: 37–47.Google Scholar
  5. Boullin DJ, Elliott JM (1979) Binding of 3H-dihydroergocryptine to alpha-adrenoceptors of intact human platelets. Br J Pharmacol Chemother 66: 89 PGoogle Scholar
  6. Braestrup C, Schmiechen R, Neef G, Nielsen M, Petersen E (1982) Interaction of convulsive ligands with benzodiazepine receptors. Science 216: 1241–1243PubMedCrossRefGoogle Scholar
  7. Curzon G (1981) The turnover of 5-hydroxytryptamine. In: Pycock CJ, Taberner PV (eds) Central neutransmitter turnover. Croom Helm, London, pp 59–90Google Scholar
  8. Gudat F, Laubscher A, Otten U, Pletscher A (1981) Shape change induced by biologically active peptides and nerve growth factors in blood platelets of rabbits. Br J Pharmacol 74: 533–538PubMedGoogle Scholar
  9. Hambley JW, Johnston GAR (1985) Uptake of gamma-aminobutyric acid by human blood platelets: comparison with CNS uptake. Life Sci 36: 2053–2058PubMedCrossRefGoogle Scholar
  10. Hefez A, Oppenheim B, Youdim MBH (1980) Human platelet aggregation response to serotonin as an index of efficacy of chlorpromazine. In: Usdin E, Sourkes TL, Youdim MBH (eds) Enzymes and neurotransmitters in mental disease. J Wiley, Chichester, pp 76–93Google Scholar
  11. Hughes CP, Berg L, Danziger WL, Cohen LA, Martin RL (1982) A new clinical scale for the staging of dementia. Br J Psychiatry 140: 566–572PubMedCrossRefGoogle Scholar
  12. Laubscher A, Pletscher A, Honegger CG, Richards JG (1979) Shape change of blood platelets brought about by myelin basic protein and other basic polypeptides. Arch Pharmacol 310: 87–92CrossRefGoogle Scholar
  13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275PubMedGoogle Scholar
  14. Mangano RM, Schwarcz R (1981) The human platelet as a model for the glutamatergic neuron: platelet uptake of L-glutamate. J Neurochem 36: 1067–1076PubMedCrossRefGoogle Scholar
  15. Marangos PJ, Campbell IC, Schmechel DE, Murphy DL, Goodwin FK (1980) Blood platelets contain a neuron-specific enolase. J Neurochem 34: 1254–1258PubMedCrossRefGoogle Scholar
  16. Marangos PJ, Patel J, Boulenger J-P, Clark-Rosenberg R (1982) Characterization of peripheral-type benzodiazepine binding sites in brain using 3H-Ro 5–4864. Mol Pharmacol 22: 26–32PubMedGoogle Scholar
  17. Mendelson W, Cain M, Cook J, Paul S, Skolnick P (1983) A benzodiazepine receptor antagonist decreases sleep and reverses the hypnotic actions of flurazepam. Science 219: 414–416PubMedCrossRefGoogle Scholar
  18. Moingeon P, Dessaux JJ, Fellous R, Alberici GF, Bidart JM, Motté P, Bohuon C (1984) Benzodiazepine receptors on human blood platelets. Life Sci 35: 2003–2009PubMedCrossRefGoogle Scholar
  19. Ninan P, Insel T, Cohen R, Cook J, Skolnick P, Paul S (1982) Benzodiazepine receptor-mediated experimental “anxiety” in primates. Science 218: 1332–1334PubMedCrossRefGoogle Scholar
  20. Paul SM, Rehavi M, Hulihan B, Skolnick P, Goodwin FK (1982) A rapid and sensitive radio-receptor assay for tertiary amine tricyclic antidepressants. Comm Psychopharmacol 22: 56–59Google Scholar
  21. Pearse AGE (1877) The diffuse neuroendocrine system and the APUD concept: related “endocrine” peptides in brain, intestine, pituitary, placenta, and anuran cutaneous glands. Med Biol 55: 115–125Google Scholar
  22. Pletscher A (1978) Platelets as models for monoaminergic neurons In: Youdim MBH (ed) Essays in neurochemistry and neuropharmacology, vol 3. John Wiley, London, pp 49–101Google Scholar
  23. Pletscher A (1988) Platelets as models: use and limitations. Experientia 44: 152–155PubMedCrossRefGoogle Scholar
  24. Rotman A (1983) Blood platelets in psychopharmacological research. Prog Neuropsychopharmacol Biol Psychiatry: 135–151Google Scholar
  25. Stahl SM (1985) Platelets as pharmacologic models for the receptors and biochemistry of monoaminergic neurons In: Longenecker GL (ed) The platelets: physiology and pharmacology. Academic Press, London, pp 307–340Google Scholar
  26. Stahl SM, Meltzer HY (1978) A kinetic and pharmacologic analysis of 5-hydroxytryptamine transport by human platelets and platelet storage granules: comparisons with central serotonergic neurons. J Pharmacol Exp Ther 205: 118–132PubMedGoogle Scholar
  27. Steer ML, Atlas DC (1982) Demonstration of human platelet beta-adrenergic receptors using 125 I-labeled cyanopindolol and 125 I-labeled hydroxybenzylpindolol. Biochim Biophys Acta 686: 240–247PubMedCrossRefGoogle Scholar
  28. Wang JKT, Taniguchi T, Spector S (1980) Properties of 3H-diazepam binding sites on rat blood platelets. Life Sci 27: 1881–1888PubMedCrossRefGoogle Scholar
  29. White HL (1979) 4-Aminobutyrate: 2-oxoglutarate aminotransferase in blood platelets. Science 205: 696–698Google Scholar
  30. Youdim MBH, Collins GGS, Sandler M, Jones AB, Pare CMB, Nicholson WJ (1972) Human brain monoamine oxidase: multiple forms and selective inhibitors. Nature 236: 225–226PubMedCrossRefGoogle Scholar
  31. Youdim MBH, Oppenheim B (1981) The effect of tryptolines (1,2,3,4-tetrahydro-betacarbolines) on monoamine metabolism and the platelet aggregation in human platelets. Neuroscience 6: 801–810PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • P. Bongioanni
    • 1
  • F. Dadone
    • 1
  • M. Donato
    • 1
  1. 1.Scuola Superiore di Studi Universitari e di Perfezionamento — “S. Anna”PisaItaly

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