The Role of Endothelial Second Messenger’s-Generating System in the Pathogenesis of Brain Oedema

Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)


The role of second messengers in the regulation of protein phosphorylation was studied in microvessels isolated from rat cerebral cortex. Calcium-calmodulin (CAM)-, Ca2+ /phospholipid (PK C)-, cyclic GMP (cGMP)-, and cyclic AMP (cAMP)-dependent protein kinases were detected. Autophosphorylation of both the α- and ß- subunits of CAM-dependent protein kinase and the proteolytic fragment of the PK C enzyme was also detected.

In other experiments, the effect of the protein kinase C enzyme inhibitor H-7 was examined on the brain oedema formation evoked by bilateral occlusion of the common carotid arteries in Sprague-Dawley rats of CFY strain.


Brain Oedema Carotid Artery Occlusion Cerebral Endothelial Cell Bilateral Occlusion Brain Oedema Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Amstrong BK, Robinson PJ, Rapoport SI (1987) Size-dependent blood-brain barrier opening demonstrated with 14C sucrose and a 200,000-Da 3H dextran. Exp Neurol 97: 686–696CrossRefGoogle Scholar
  2. 2.
    Dux E, Temesvari P, Szerdahelyi P, Nagy A, Kovacs J. Joó F (1987) Protective effect of antihistamines on cerebral oedema induced by experimental pneumothorax in newborn piglets. Neuroscience 22: 317–321PubMedCrossRefGoogle Scholar
  3. 3.
    Joó F, Karnushina I (1973) A procedure for the isolation of capillaries from rat brain. Cytobios 8: 41–48PubMedGoogle Scholar
  4. 4.
    Joó F, Tósaki A, Olah Z, Koltai M (1989) Inhibition by H-7 of the protein kinase C prevents formation of brain oedema in Sprague-Dawley CFY rats. Brain Res 490: 141–143PubMedCrossRefGoogle Scholar
  5. 5.
    Karnushina I, Palacios JM, Barbin G, Dux E, Joó F, Schwartz JC (1980) Studies on a capillary-rich fraction isolated from brain: histaminic components and characterization of the histamine receptors linked to adenylate cyclase. J Neurochem 34: 1201–1208PubMedCrossRefGoogle Scholar
  6. 6.
    Karnushina I, Tóth I, Dux E, Joó F (1980) Presence of the guanylate cyclase in brain capillaries: histochemical and biochemical evidences. Brain Res 189: 588–592PubMedCrossRefGoogle Scholar
  7. 7.
    Mackie K, Lai Y, Nairn AC, Greengard P. Pitt BR, Lazo JS (1986) Protein phosphorylation in cultured endothelial cells. J Cell Physiol 128: 367–374PubMedCrossRefGoogle Scholar
  8. 8.
    Olah Z. Novak R, Lengyel I, Dux E, Joó F (1988) Kinetics of protein phosphorylation in microvessels isolated from rat brain: modulation by second messengers. J Neurochem 51: 49–56PubMedCrossRefGoogle Scholar
  9. 9.
    Tósaki A, Koltai M, Joó F, Adam G, Szerdahelyi P, Lepran I, Takats I, Szekeres L (1985) Actinomycin D suppresses the protective effect of dexamethasone in rats affected by global cerebral ischaemia. Stroke 16: 501–505PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • F. Joó
    • 1
  1. 1.Laboratory of Molecular Neurobiology, Institute of BiophysicsBiological Research CenterSzegedHungary

Personalised recommendations