The Role of Endothelial Second Messenger’s-Generating System in the Pathogenesis of Brain Oedema
Part of the
book series (NEUROCHIRURGICA, volume 51)
The role of second messengers in the regulation of protein phosphorylation was studied in microvessels isolated from rat cerebral cortex. Calcium-calmodulin (CAM)-, Ca2+ /phospholipid (PK C)-, cyclic GMP (cGMP)-, and cyclic AMP (cAMP)-dependent protein kinases were detected. Autophosphorylation of both the α- and ß- subunits of CAM-dependent protein kinase and the proteolytic fragment of the PK C enzyme was also detected.
In other experiments, the effect of the protein kinase C enzyme inhibitor H-7 was examined on the brain oedema formation evoked by bilateral occlusion of the common carotid arteries in Sprague-Dawley rats of CFY strain.
KeywordsBrain Oedema Carotid Artery Occlusion Cerebral Endothelial Cell Bilateral Occlusion Brain Oedema Formation
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Amstrong BK, Robinson PJ, Rapoport SI (1987) Size-dependent blood-brain barrier opening demonstrated with 14C sucrose and a 200,000-Da 3H dextran. Exp Neurol 97: 686–696CrossRefGoogle Scholar
Dux E, Temesvari P, Szerdahelyi P, Nagy A, Kovacs J. Joó F (1987) Protective effect of antihistamines on cerebral oedema induced by experimental pneumothorax in newborn piglets. Neuroscience 22: 317–321PubMedCrossRefGoogle Scholar
Joó F, Karnushina I (1973) A procedure for the isolation of capillaries from rat brain. Cytobios 8: 41–48PubMedGoogle Scholar
Joó F, Tósaki A, Olah Z, Koltai M (1989) Inhibition by H-7 of the protein kinase C prevents formation of brain oedema in Sprague-Dawley CFY rats. Brain Res 490: 141–143PubMedCrossRefGoogle Scholar
Karnushina I, Palacios JM, Barbin G, Dux E, Joó F, Schwartz JC (1980) Studies on a capillary-rich fraction isolated from brain: histaminic components and characterization of the histamine receptors linked to adenylate cyclase. J Neurochem 34: 1201–1208PubMedCrossRefGoogle Scholar
Karnushina I, Tóth I, Dux E, Joó F (1980) Presence of the guanylate cyclase in brain capillaries: histochemical and biochemical evidences. Brain Res 189: 588–592PubMedCrossRefGoogle Scholar
Mackie K, Lai Y, Nairn AC, Greengard P. Pitt BR, Lazo JS (1986) Protein phosphorylation in cultured endothelial cells. J Cell Physiol 128: 367–374PubMedCrossRefGoogle Scholar
Olah Z. Novak R, Lengyel I, Dux E, Joó F (1988) Kinetics of protein phosphorylation in microvessels isolated from rat brain: modulation by second messengers. J Neurochem 51: 49–56PubMedCrossRefGoogle Scholar
Tósaki A, Koltai M, Joó F, Adam G, Szerdahelyi P, Lepran I, Takats I, Szekeres L (1985) Actinomycin D suppresses the protective effect of dexamethasone in rats affected by global cerebral ischaemia. Stroke 16: 501–505PubMedCrossRefGoogle Scholar