Ischaemic Brain Oedema and Xanthine-Xanthine Oxidase System

  • Y. Kinuta
  • H. Kikuchi
  • M. Ishikawa
Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)


The formation of oxygen-derived free radicals in cerebral ischaemia has been implicated in altering the BBB permeability, cause oedema and tissue damage. However little attention has been paid regarding the involvement of xanthine oxidase in the cerebral ischaemic events. Recently we demonstrated that cerebral ischaemia promotes the conversion of xanthine oxidase type D (nicotinamide adenine dinucleotide — dependent dehydrogenase) to type 0 (oxygen — dependent superoxide — producing oxidase). This investigation was concerned with elucidating the relationship between the conversion of xanthide oxidase and the duration of brain ischaemia. Four vessel-occlusion served as a model for the induction of cerebral ischaemia in rats. Xanthine oxidase was assayed by high pressure liquid chromatography using ultraviolet and electrochemical detection. The enzymatic conversion of xanthine oxidase from type D to type O increased with time from 7.6–15% during 5min ischaemia to 27% and 36% at 15 min and 30 min after ischaemia, respectively. These results support the contention that xanthine oxidase may participate in free radical-induced ischaemic brain oedema.


Cerebral Ischaemia Xanthine Oxidase Nicotinamide Adenine Dinucleotide Enzymatic Conversion 5min Ischaemia 
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  1. 1.
    Chan PH, Schmidley JW, Fishman RA et al (1984) Brain injury, oedema, and vascular permeability changes induced by oxygen-derived free radicals. Neurology 34: 315–320PubMedGoogle Scholar
  2. 2.
    Della Corte E, Stirpe F (1972) The regulation of rat liver xanthine oxidase. Biochem J 126: 739–745PubMedGoogle Scholar
  3. 3.
    Kinuta Y, Kimura M, Itokawa Y et al (1989) Changes in xanthine oxidase in ischaemic rat brain. J Neurosurg 71: 417–420PubMedCrossRefGoogle Scholar
  4. 4.
    Kinuta Y, Kikuchi H, Ishikawa M et al (1989) Lipid peroxidation in focal cerebral ischaemia. J Neurosurg 71: 421–429PubMedCrossRefGoogle Scholar
  5. 5.
    Podzuweit T, Braun W, Muller A et al (1987) Arrhythmias and infarction in the ischaemic pig heart are not mediated by xanthine oxidase-derived free oxygen radicals. Basic Res Cardiol 82: 493–505PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. Kinuta
    • 1
  • H. Kikuchi
    • 1
  • M. Ishikawa
    • 1
  1. 1.Department of NeurosurgeryKyoto UniversityKyotoJapan

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