Inducible Ornithine Decarboxylase Expression in Brain Subject to Vasogenic Oedema After Transient Ischaemia: Relationship to C-fos Gene Expression

  • R. J. Dempsey
  • J. M. Carney
  • M. S. Kindy
Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)


Ornithine Decarboxylase (ODC) is the rat controlling enzyme of polyamine biosynthesis and has been shown to be produced in a delayed fashion in response to cerebral ischaemia. Its appearance has been linked to the development of vasogenic brain oedema. To understand the genetic control of this protein, Mongolian gerbils were studied for the possible expression of the ODC gene as compared to that of the inducible proto oncogenes c-fos and c-jun after transient bilateral carotid artery occlusion.

Total cellular RNA was isolated from gerbil brains by guanidinethiocyanate extraction and characterized by northern blot analysis for c-fos, c-jun, and ODC mRNA over reperfusion times. c-fos and c-jun expression rose rapidly with peak level reached at 60 min of reperfusion (70 × control, p ≤ 0.01). Peak levels of ODC mRNA induction were seen at 4hrs reperfusion (2.83 × control, p ≤ 0.01) consistent with the period of maximum of brain oedema as measured by specific gravity (1.0386 ± 0.0009, p ≤ 0.05). These data indicate the differential timing of genetic expression during the reperfusion period after transient ischaemia. Such studies suggest that potential therapies may be possible by addressing the delayed ODC component of ischaemic oedema formation and allow a greater understanding of the role of gene induction in the multifaceted cerebral response to ischaemia.


Cerebral Ischaemia Brain Oedema Ornithine Decarboxylase Vasogenic Oedema Mongolian Gerbil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Dempsey RJ, Roy MW, Meyer K, Tai H, Olsen J (1985) Polyamine and prostaglandin markers in focal cerebral ischaemia. Neurosurgery 17: 635–640PubMedCrossRefGoogle Scholar
  2. 2.
    Dempsey RJ, Combs DJ, Olson JW, Maley M (1988) Brain ornithine decarboxylase activity following transient cerebral ischaemia — Relationship to cerebral oedema development. Neurol Res 10: 175–178PubMedGoogle Scholar
  3. 3.
    Klatzo I, Suzuki R, Orzi F et al (1982) Pathomechanisms of ischaemic brain oedema. In: Go KG, Baethmann A (eds) Recent progress in the study of brain oedema. Plenum Press, New York and LondonGoogle Scholar
  4. 4.
    Koenig H, Goldstone A, Lu Cy (1983) Blood-brain barrier breakdown in brain oedema following cold injury is mediated by microvascular polyamines. Biochem Biophys Res Comm 116: 1039–1048PubMedCrossRefGoogle Scholar
  5. 5.
    Marmarou A, Tanaka K, Shulman S (1987) An improved grayimetric measure of oedema. J Neurosurg 49: 530–537Google Scholar
  6. 6.
    Mitchell RL, Hanks SK, Verma IM (1987) Proto-oncogene fos: An inducible multifaceted gene. Symposium on Fundamental Cancer Research vol 39Google Scholar
  7. 7.
    Schmitz MP, Combs DJ, Dempsey RJ: Difluoromethylornithine (DFMO) inhibition of polyamine pathway synthesis decreases post-ischaemic oedema and blood-brain barrier (BBB) breakdown. Personal CommunicationGoogle Scholar
  8. 8.
    Smeal T, Angel P, Meek J, Karin M (1989) Different requirements for formation of Jun: Jun and Jun: Fos complexes. Genes & Development 3: 2091–2100CrossRefGoogle Scholar
  9. 9.
    Trout JJ, Koening H, Goldstone A, Lu Cy (1986) Blood-brain barrier breakdown by cold injury, polyamine signals mediate acute stimulation of endocytosis, vesicular transport, microvillus formation in rat cerebral capillaries. Lab Invest 55: 622–631PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • R. J. Dempsey
    • 1
  • J. M. Carney
    • 1
  • M. S. Kindy
    • 1
  1. 1.University of Kentucky Medical Center and Veterans Administration HospitalLexingtonUSA

Personalised recommendations