Summary
Numerous neuropathological conditions are accompanied by astroglial swelling which may contribute to secondary neuronal damage. From 4–27 days in vitro,swelling of astroglial cells was induced by hypo-osmolar solutions and high K+ solutions and the cells were examined by microscopy and flow cytometry. Regulatory volume decrease was observed beginning after approximately 30 minutes and was accompanied by increased membrane activity. Arginine vasopressin caused gradual persistent astroglial swelling. Atrial natriuretic peptide caused retraction of the astroglial cell processes but did not significantly alter the swelling induced by hypo-osmolar or elevated K+ solutions. The membrane mechanisms responsible for the control of astroglial cell volume did not appear to be specific for mature astroglia, rather they are present at all developmental stages.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Beaumont K, Tan PK (1990) Effects of atrial and brain natriuretic peptides upon cyclic GMP levels, potassium transport, and receptor binding in rat astrocytes. J Neurosci Res 25: 256–262
Bender AS, Hertz L (1987) Inhibition of [3H]diazepam binding in primary cultures of astrocytes by atrial natriuretic peptide and by a cyclic GMP analog. Brain Res 436: 189–192
Cholewinski AJ, Wilkin GP (1988) Peptide receptors on astrocytes: evidence for regional heterogeneity. Biochem Soc Trans 16: 429–432
Doczi T, Joo F, Szerdahelyi P, Bodosi M (1988) Regulation of brain water and electrolyte contents: the opposite actions of central vasopressin and atrial natriuretic factor ( ANF ). Acta Neurochir (Wien) [Suppl] 43: 186–188
Fedoroff S, Neal J, Opas M, Kalnins VI (1984) Astrocyte cell lineage. III. The morphology of differentiating mouse astrocytes in colony culture. J Neurocytol 13: 1–20
Kimelberg HK, Ransom BR (1986) Physiological and pathological aspects of astrocytic swelling. In: Fedoroff S, Vernadakis A (eds) Astrocytes, vol 3. Academic Press, Orlando FL, pp 129–166
McGann LE, Walterson ML, Hogg LM (1988) Light scattering and cell volumes in osmotically stressed and frozen-thawed cells. Cytometry 9: 33–38
Walz W (1987) Swelling and potassium uptake in cultured astrocytes. Can J Physiol Pharmacol 65: 1051–1057
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag
About this paper
Cite this paper
Del Bigio, M.R., Fedoroff, S. (1990). Swelling of Astroglia in vitro and the Effect of Arginine Vasopressin and Atrial Natriuretic Peptide. In: Reulen, HJ., Baethmann, A., Fenstermacher, J., Marmarou, A., Spatz, M. (eds) Brain Edema VIII. Acta Neurochirurgica, vol 51. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9115-6_5
Download citation
DOI: https://doi.org/10.1007/978-3-7091-9115-6_5
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9117-0
Online ISBN: 978-3-7091-9115-6
eBook Packages: Springer Book Archive