Oxygen Free Radicals in the Genesis of Peritumoural Brain Oedema in Experimental Malignant Brain Tumours

  • Y. Ikeda
  • D. M. Long
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)


Disruption of blood brain barrier with increased vascular permeability is associated with genesis of peritumoural oedema. Oxygen free radicals play a role in increased vascular permeability. Recent studies have suggested that tumour cells can produce superoxide radicals and free radical scavengers such as superoxide dismutase (SOD) and catalase in tumour cells are impaired. In this study, we investigated the role of oxygen free radicals in the genesis of peritumoural brain oedema in experimental malignant brain tumours using V × 2 carcinoma cells and 9L glioma cells. In vitro data indicate that the V × 2 carcinoma cell and the 9L glioma cells produce superoxide radicals detected by nitroblue tetrazolium. Electron spin resonance spectroscopy using DMPO as a spin trap demonstrated that SOD activity was significantly lower in subcutaneous larger 9L glioma tumours than in normal brains and 9L glioma brain tumours.

In the subcutaneous tumours, SOD activity was lower in the central portion of the tumour than in the peripheral portion of the tumour.

In conclusion, we are not sure whether oxygen free radicals are major causative factors of peritumoural brain oedema, but the demonstration of oxygen free radicals in brain tumour cells needs further investigation.


Electron Spin Resonance Brain Tumour Brain Oedema Oxygen Free Radical Spin Trap 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Ikeda Y, Anderson JH, Long DM (1989) Oxygen free radicals in the genesis of traumatic and peritumoural brain oedema. Neurosurgery 24: 679–685PubMedCrossRefGoogle Scholar
  2. 2.
    Long DM (1979) Capillary ultrastructure in human metastatic brain tumours. J Neurosurg 51: 53–58PubMedCrossRefGoogle Scholar
  3. 3.
    Sun Yi, Oberley LW, Elwell JH, Sierra-Rivera E (1989) Antioxidant enzyme activities in normal and transformed mouse liver cells. Int J Cancer 44: 1028–1033CrossRefGoogle Scholar
  4. 4.
    Oberley LW, Buettner GR (1979) Role of superoxide dismutase in cancer: A review. Cancer Res 39: 1141–1149PubMedGoogle Scholar
  5. 5.
    Hiramatsu M, Kohno M (1987) Determination of superoxide dismutase activity by electron spin resonance spectrometry using the spin trap method. Jeol News 23: 7–9Google Scholar
  6. 6.
    Halliwell B, Gutteridge JMC (1984) Review article. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219: 1–14PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. Ikeda
    • 2
  • D. M. Long
    • 1
  1. 1.Johns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Department of NeurosurgeryNippon Medical SchoolBunkyo-ku, Tokyo 113Japan

Personalised recommendations