Routed Protein Migration After Protein Extravasation and Water Leakage Caused by Cold Injury
The movement of extravasated endogenous protein induced by cold injury was investigated in the rat. Extravasated proteins were observed around the cold injury immediately after injury; within 3 hr they had moved into the ipsi- and contralateral hemispheres along the nerve fibers (routed protein migration). The water content in the areas where the extravasated protein was observed was increased, and NMR-CT scans (TR 2000 msec, TE 90 msec) showed high intensity patterns whose distribution and progression coincided with those of extravasated protein. NMR relaxation time, T1, showed a slight increase in the area where the extravasated protein was observed, but T2 value did not show any significant changes. In the opposite hemisphere, CBF decreased within 3 to 6 hours after injury. Local cerebral glucose utilization was reduced, but this change occurred more than 6 hours after injury. These results indicate that water leakage and protein extravasation occur simultaneously after the injury without any significant change of water state or protein conformation. Subsequently, a reduction of CBF is induced, which is followed by changes in cerebral metabolism.
KeywordsCerebral Blood Flow Contralateral Hemisphere Cereb Blood Flow Water Leakage Cold Injury
Unable to display preview. Download preview PDF.
- 1.Houthoff HJ, Go KG (1980) Endogenous versus exogenous protein tracer passage in blood-brain barrier damage. In: CervosNavarro J, Ferszt R (eds) Advances in neurology, Vol 28: Brain oedema. Raven Press, New York, pp 75–80Google Scholar
- 2.Shinohara Y, Izumi S, Watanabe K (1981) Evaluation of BBB function and movement of protein in cold injury by anti-peroxidase immunostaining method. J Cereb Blood Flow Metab 1 [Suppl 1]: pp 393–394Google Scholar
- 3.Shinohara Y, Takagi S, Yoshii F, Tanaka M, Izumi S, Watanabe K (1983) Movement of extravasated protein and changes of CBF and cerebral glucose utilization in BBB dysfunction in rats. J Cereb Blood Flow Metab 3 [Suppl 1]: pp 437–438Google Scholar
- 5.Shinohara Y, Ohsuga H, Takizawa S, Haida M, Saitoh S, Taniguchi R (1987) Routed protein migration followed by changes of water content and water states. In: Meyer JS et al (eds) Cerebral vascular disease 6. Elsevier Science Publishers B.V., Excerpta Medica, Amsterdam New York OxfordGoogle Scholar
- 6.Shinohara Y, Ohsuga H, Tanaka M, Takizawa S, Izumi S, Watanabe K (to be submitted) Evaluation of blood-brain barrier function by anti-horseradish peroxidase immunostaining method and comparison with horseradish peroxidase-labelled antibody methodGoogle Scholar