Routed Protein Migration After Protein Extravasation and Water Leakage Caused by Cold Injury
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The movement of extravasated endogenous protein induced by cold injury was investigated in the rat. Extravasated proteins were observed around the cold injury immediately after injury; within 3 hr they had moved into the ipsi- and contralateral hemispheres along the nerve fibers (routed protein migration). The water content in the areas where the extravasated protein was observed was increased, and NMR-CT scans (TR 2000 msec, TE 90 msec) showed high intensity patterns whose distribution and progression coincided with those of extravasated protein. NMR relaxation time, T1, showed a slight increase in the area where the extravasated protein was observed, but T2 value did not show any significant changes. In the opposite hemisphere, CBF decreased within 3 to 6 hours after injury. Local cerebral glucose utilization was reduced, but this change occurred more than 6 hours after injury. These results indicate that water leakage and protein extravasation occur simultaneously after the injury without any significant change of water state or protein conformation. Subsequently, a reduction of CBF is induced, which is followed by changes in cerebral metabolism.
KeywordsCerebral Blood Flow Contralateral Hemisphere Cereb Blood Flow Water Leakage Cold Injury
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