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Mechanisms Underlying Glutamate-induced Swelling of Astrocytes in Primary Culture

  • P. H. Chan
  • L. Chu
Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)

Summary

The effects of glutamate and its agonists and antagonists on the swelling of primary astrocytes were studied. Glutamate, aspartate, homocysteate, and quisqualate caused a significant increase in astrocytic swelling as measured by 3-0-methyl-[14C]-glucose uptake and by phase-contrast microscopic observations. N-methyl-D-aspartate and kainate were not effective, nor were the competitive NMDA receptor antagonists. Ketamine and MK-801, the non-competitive NMDA receptor antagonists protected the cultured astrocytes against glutamate-induced swelling. Moreover, Glu-induced astrocytic swelling was significantly reduced when sodium-ions were depleted from the culture medium. The sodium ion dependence of Glu-induced astrocytic swelling is rather specific, since depletion of Ca2+ or Mg2+ had no effect. Our data suggest that Na+-dependent, Glu-mediated cell swelling occurs in primary cell cultures of astrocytes.

Keywords

NMDA Receptor NMDA Receptor Antagonist Primary Cell Culture Primary Astrocyte Noncompetitive NMDA Receptor Antagonist 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • P. H. Chan
    • 1
  • L. Chu
    • 1
  1. 1.CNS Injury and Edema Center, Department of Neurological Surgery and NeurologySchool of Medicine, University of CaliforniaSan FranciscoUSA

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