Comparative Effects of Direct and Indirect Hydroxyl Radical Scavengers on Traumatic Brain Oedema

  • Y. Ikeda
  • D. M. Long
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)


The effects of dimethyl sulfoxide (DMSO) and dimethylthiourea (DMTU) and deferoxamine (DFO) were investigated in cats during a standard cortical freezing method for the production of vasogenic brain oedema. Hydroxyl radical scavenging activity was studied by electron spin resonance assay using DMPO as the trap. The hydroxyl radical spin adduct was extinguished by DFO and a large dose of DMTU. Evans blue dye extravasation was reduced in DFO and DMSO treated groups as well. DFO appears to be an effective agent for the prevention of cold induced oedema. DMSO and DMTU are direct hydroxyl radical scavengers. These studies suggest that scavenging the hydroxyl radicals has a beneficial effect in the treatment of traumatic brain oedema.


Brain Oedema Oxygen Free Radical Evans Blue Hydroxyl Radical Scavenger Hydroxyl Radical Scavenge Activity 


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  1. 1.
    Bulkley GB (1987) Free radicall-mediated reperfusion injury: a selective review: Br J Cancer [Suppl] 8: 66–73Google Scholar
  2. 2.
    Ikeda Y, Anderson JH, Long DM (1989) Oxygen free radicals in the genesis of traumatic and peritumoral brain oedema. Neurosurgery 24: 679–685PubMedCrossRefGoogle Scholar
  3. 3.
    Halliwell B, Gutteridge JMC (1986) Oxygen free radicals and iron in relation to biology and medicine: Some problems and concepts. Arch Biochem Biophys 246: 501–514Google Scholar
  4. 4.
    Ikeda Y, Ikeda K, Long DM (1989) Protective effect of the iron chelator deferoxamine on cold-induced brain oedema. J Neurosurg 71: 233–238PubMedCrossRefGoogle Scholar
  5. 5.
    Bolli R, Zhu W (1987) Hartley CJ, Michael LH, Repine JB, Hess ML, Kukreja RC, Roberts R. Attenuation of dysfunction in the postischemic stunned myocardium by dimethylthiourea. Circulation 76: 458–468Google Scholar
  6. 6.
    Fox RB (1984) Prevention of granulocyte-mediated oxidant injury in rats by a hydroxyl radical scavenger, dimethylthiourea. J Clin Invest 7: 1456–1464CrossRefGoogle Scholar
  7. 7.
    Parker NB, Bergen BM, Curtis WE, Muldraw ME, Linas SL, Repine JE (1985) Hydrogen peroxidase causes dimethylthiourea consumption while hydroxyl radical casuses dimethyl sulfoxide consumption in vitro. J Free Radical Biol Med 1: 415–419CrossRefGoogle Scholar
  8. 8.
    Imlay JA, Chin SM, Linn S (1987) Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro. Science 240: 640–642CrossRefGoogle Scholar
  9. 9.
    Ikeda Y, Ikeda K, Long DM (1989) Comparative study of different iron-chelating agents in cold-induced brain oedema. Neurosurgery 24: 820–824PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. Ikeda
    • 1
  • D. M. Long
    • 2
  1. 1.Department of NeurosurgeryNippon Medical SchoolTokyoJapan
  2. 2.Department of NeurosurgeryThe Johns Hopkins HospitalBaltimoreUSA

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