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Effects of Antineutrophil Serum (ANS) on Posttraumatic Brain Oedema in Rats

  • L. Schürer
  • U. Prügner
  • O. Kempski
  • K.-E. Arfors
  • A. Baethmann
Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)

Summary

The role of white blood cells in acute central nervous system disorders, such as stroke or traumatic injury is poorly understood. In this experimental study the effect of neutropenia on posttraumatic brain oedema was investigated. Polyclonal antiserum against polymorphonuclear leukocytes was used to induce neutropenia. Control animals received normal serum or no treatment at all. Hemispheric swelling and brain water content after cryo-injury to the exposed left cerebral hemisphere was gravimetrically assessed, and by the wet weight-dry weight method. Administration of anti-neutrophil serum led to a significant reduction of circulating neutrophils. In untreated animals or in rats receiving normal serum, hemispheric swelling of the brain was 7.26 ± 0.35% or 8.52 ± 0.26%, respectively. Neutropenia induced by anti-neutrophil serum resulted in a significant increase in hemispheric swelling to 11.44 ± 0.84% (p < 0.001). It may be noted, however, that the enhancement of brain swelling by neutropenia was not obvious when comparing the water contents of the affected hemispheres of animals subjected to cold injury with and without anti-neutrophil serum.

The enhancement of brain oedema by neutropenia suggests a protective function of neutrophils against extravasation in the presence of a broken blood-brain barrier.

Keywords

Brain Oedema Polyclonal Antiserum Brain Water Content Cold Injury Nervous System Disorder 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hernandez LA, Grisham MB, Twohig B, Arfors K-E, Harlan JM, Granger DN (1987) Role of neutrophils in ischaemia-reperfusion-induced microvascular injury. Am J Physiol 253: H699 - H703PubMedGoogle Scholar
  2. 2.
    Sandler H, Högstrop H, Lundberg C, Gerdin B (1987) Antiserum-induced neutropenia in the rat: characterization of a rabbit anti-rat neutrophil serum. Br J Exp Pathol 68: 71–80PubMedGoogle Scholar
  3. 3.
    Schoettle RJ, Kochanek PM, Nemoto EM, Barmada MA, Magaree MJ, Melick JA (1989) Granulocyte accumulation and oedema after cerebral trauma. Crit Care Med 17: S71Google Scholar
  4. 4.
    Unterberg A, Wahl M, Hammersen F, Baethmann A (1987) Permeability and vasomotor response of cerebral vessels during exposure to arachidonic acid. Acta Neuropathol (Berl) 73: 209–219CrossRefGoogle Scholar
  5. 5.
    Weiss SJ (1989) Tissue destruction by neutrophilys. New Engl J Med 320: 365–375PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • L. Schürer
    • 2
  • U. Prügner
    • 1
  • O. Kempski
    • 1
  • K.-E. Arfors
    • 1
  • A. Baethmann
    • 1
  1. 1.Institut of Surgical Research, Klinikum GroßhadernLudwig-Maximilians-Universität MünchenMünchenFederal Republic of Germany
  2. 2.Institut for Surgical Research, Klinikum GroßhadernLudwig-Maximilians-Universität MünchenMünchen 70Federal Republic of Germany

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