Summary
Continuous detection of the permeability changes of the blood-brain (BB) interface became feasible with the recent development of the cerebral microdialysis technique6.
Vasogenic brain oedema was induced by the insertion of the dialysis capillary into the cerebral cortex of rats, and blood-brain transfer was measured with Cr51-EDTA as a blood-born tracer. The dialysis capillaries had an upper limit of 500 daltons (MW). Ringer solution was perfused through the dialysis tube. Samples were collected continuously. The permeability coefficient has been calculated from the radioactivity of the serum and of the washing fluid.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Benveniste H, Hansen AJ, Ottosen NS (1989) Determination of Brain Interstitial Concentrations by Microdialysis. J Neurochem 52: 1741–1750
Fenstermacher J, Blasberg R, Patlak C (1981) Methods for quantifying the transport of drugs across brain barrier systems. Pharmacol Ther 14: 217–248
Ohno K, Pettigrew K, Rapoport S (1978) Lower limits of cerebrovascular permeability to non-electrolytes in the conscious rat. Am J Physiol 33: 635–645
Persson L, Hansson HA, Sourander P (1976) Extravasation, pread and cellular uptake of Evans blue-labelled albumin around a reproducible small stab-wound in the rat brain. Acta Neuropoath (Berlin) 34: 125–136
Phebus LA, Perry KW, Clemenc JA, Fuller RW (1986) Brain anoxia releases striatal dopoamine in rats. Life Sci 38: 2447–2453
Ungerstedt U, Herrera-Marchintz M, Jungnelius U, Stahle L, Tossman U, Zetterstrom T (1982) Dopamine synaptic mechanisms reflected in studies combining behavioural recordings and spread and cellular uptake of Evans blue-labelled albumin around brain dialysis. In: Kotisaka M (ed) Advances in dopamine rea reproducible small stab-wound in the rat brain. Acta Neuro- search. Pergamon Press, New York, pp 219–231
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag
About this paper
Cite this paper
Major, O., Shdanova, T., Duffek, L., Nagy, Z. (1990). Continuous Monitoring of Blood-Brain Barrier Opening to Cr51-EDTA by Microdialysis Following Probe Injury. In: Reulen, HJ., Baethmann, A., Fenstermacher, J., Marmarou, A., Spatz, M. (eds) Brain Edema VIII. Acta Neurochirurgica, vol 51. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9115-6_16
Download citation
DOI: https://doi.org/10.1007/978-3-7091-9115-6_16
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9117-0
Online ISBN: 978-3-7091-9115-6
eBook Packages: Springer Book Archive