A New Mapping Study of Superoxide Free Radicals, Vascular Permeability and Energy Metabolism in Central Nervous System

  • N. Hayashi
  • T. Tsubokawa
  • B. A. Green
  • B. D. Watson
  • R. Prado
Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 51)


To study the role of free radicals in various pathophysiologies in the central nervous system (CNS), we have introduced a new simultaneous mapping technique of superoxide free radicals, vascular permeability and energy metabolism. The detection of superoxide anion in the CNS is based on the 380 nm chemiluminescence of 2-methyl6-phenyl-3,7-dihydroimidazo [1,2-alpyrazin-3-one(CLA-phenyl)] upon reaction with superoxide in the frozen tissue section. The results show topographical relationships between activation of superoxide free radicals, increased vascular permeability, and changes of hydrogen transport system in the energy metabolism (Fig. 2). Prolonged formation of superoxide free radicals in the white matter without vasogenic oedema and necrotic tissue are interesting as a mechanism of progression of secondary tissue damage in ischaemic insults.


Vascular Permeability Cereb Blood Flow Freeze Tissue Section Spinal Cord Infarction Superoxide Free Radical 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • N. Hayashi
    • 3
  • T. Tsubokawa
    • 1
  • B. A. Green
    • 2
  • B. D. Watson
    • 2
  • R. Prado
    • 2
  1. 1.Department of Neurological SurgeryNihon UniversityTokyoJapan
  2. 2.Department of Neurological SurgeryUniversity of MiamiUSA
  3. 3.Department of Neurological SurgeryNihon UniversityItasbashiku, Tokyo 173Japan

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