Turnover of monoamine oxidase B (MAO-B) in pig brain by positron emission tomography using 11C-L-deprenyl
Positron emission tomography (PET) was applied to investigate the rate of turnover of pig brain MAO-B. For this purpose 11 C-L-deprenyl was used as an irreversible ligand, which bind stoichiometrically to the enzyme. A tracer dose of11 C-L-deprenyl was injected and PET scans performed to obtain baseline deprenyl binding. A high dose of unlabelled deprenyl was then administered to inhibit the enzyme and tracer doses of 11 C-L-deprenyl, with subsequent PET analyses, were given at 0, 2, 7, 21 and 42 days. The half-life for the turnover rate calculated was found to be 6.5 days.
KeywordsPositron Emission Tomography Positron Emission Tomography Scan Tracer Dose Brain Monoamine Oxidase Liver Monoamine Oxidase
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