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Human Caki-1 cells are the first model for extraneuronal transport of noradrenaline (uptake2) which is based on a clonal cell line

  • E. Schömig
  • J. Babin-Ebell
  • C.-L. Schönfeld
  • H. Russ
  • U. Trendelenburg
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)

Summary

The neurotransmitter noradrenaline is inactivated by active transport out of the synaptic cleft — either back into the adrenergic neuron or into extraneuronal cells. Transport studies on isolated cells provide many advantages. However, an experimental model for the extraneuronal uptake of noradrenaline which is based on a clonal cell line was not known until now. The human renal carcinoma cell line Caki-1 is the first clonal cell line known to express the extraneuronal transport system for noradrenaline.

Keywords

Synaptic Cleft Clonal Cell Line Rabbit Aorta Adrenergic Neuron Opossum Kidney 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bönisch H, Bryan LJ, Henseling M, O’Donnell SR, Stockmann P, Trendelenburg U (1985) The effect of various ions on uptake2 of catecholamines. Naunyn-Schmiedebergs Arch Pharmacol 328:407–416.PubMedCrossRefGoogle Scholar
  2. Gillespie JS (1976) Extraneuronal uptake of catecholamines in smooth muscle and connective tissue. In: Paton DM (ed) The mechanism of neuronal and extraneuronal transport of catecholamines. Raven Press, New York, pp 325–354.Google Scholar
  3. Graefe K-H, Zeitner C-J, Fuchs G, Keller B (1984) Role played by sodium in the membrane transport of 3H-noradrenaline across the axonal membrane of adrenergic neurones. In: Fleming WW, Graefe K-H, Langer SZ, Weiner N (eds) Neuronal and extraneuronal events in autonomic pharmacology. Raven Press, New York, pp 51–62.Google Scholar
  4. Greene (1976) The kinetics of enzyme action and inhibition in intact tissues and tissue slices, with special reference to cholinesterase. J Pharm Pharmacol 28:265–274.Google Scholar
  5. Sanchez Armass S, Orrego F (1977) A major role for chloride in 3H-noradrenaline transport by rat heart adrenergic nerves. Life Sci 20:1829–1838.PubMedCrossRefGoogle Scholar
  6. Schömig E, Schönfeld CL (1990) Extraneuronal noradrenaline transport (uptake2) in a clonal cell line (Caki-1 cells). Naunyn-Schmiedebergs Arch Pharmacol 341:404–410.PubMedGoogle Scholar
  7. Trendelenburg U (1988) The extraneuronal uptake and metabolism of catecholamines. In: Trendelenburg U, Weiner N (eds) Handb exp pharmacol 90/I:279-319.Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • E. Schömig
    • 1
  • J. Babin-Ebell
    • 1
  • C.-L. Schönfeld
    • 1
  • H. Russ
    • 1
  • U. Trendelenburg
    • 1
  1. 1.Department of PharmacologyUniversity of WürzburgWürzburgFederal Republic of Germany

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