The importance of plasma 3,4-dihydroxyphenylglycol (DOPEG) in analyses of the sympathetic nervous system in vivo

  • K.-H. Graefe
  • Th. Halbrügge
  • M. Gerlich
  • J. Ludwig
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)


In the anaesthetized rabbit, plasma DOPEG was used as a tool to estimate both, the total-body rate of neuronal re-uptake of noradrenaline (NA) and the factor F by which the NA concentration in the synaptic cleft exceeds that in plasma. The NA re-uptake rate was 3.5 times higher than the rate of NA net release into plasma and amounted to 863 pmol kg-1 min-1 F was 3.4.

In a study in humans it was found that essential hypertension appears to be associated with an enhanced formation of that part of DOPEG which originates from NA leaking out of the transmitter storage vesicles.


Essential Hypertension Plasma Clearance Synaptic Cleft Neuronal Uptake Anaesthetize Rabbit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Eisenhofer G, Ropchak TG, Stull RW, Goldstein DS, Keiser HR, Kopin IJ (1987) Dihydroxyphenylglycol and intraneuronal metabolism of endogenous and exogenous norepinephrine in the rat vas deferens. J Pharmacol Exp Ther 241:547–553.PubMedGoogle Scholar
  2. Eisenhofer G, Goldstein DS, Kopin IJ (1989) Plasma dihydroxyphenylglycol for estimation of noradrenaline neuronal re-uptake in the sympathetic nervous system in vivo. Clin Sci 76:171–182.PubMedGoogle Scholar
  3. Eisenhofer G, Cox HS, Esler MD (1990) Parallel increases in noradrenaline reuptake and release into plasma during activation of the sympathetic nervous system in rabbits. Naunyn-Schmiedebergs Arch Pharmacol 342:328–335.PubMedGoogle Scholar
  4. Goldstein DS, Zimlichman R, Stull R, Keiser HR, Kopin IJ (1986) Estimation of intrasynaptic norepinephrine concentrations in humans. Hypertension 8:471–475.PubMedGoogle Scholar
  5. Goldstein DS, Eisenhofer G, Stull R, Folio CJ, Keiser HR, Kopin IJ (1988) Plasma dihydroxyphenylglycol and the intraneuronal disposition of norepinephrine in humans. J Clin Invest 81:213–220.PubMedCrossRefGoogle Scholar
  6. Graefe K-H, Henseling M (1983) Neuronal and extraneuronal uptake and metabolism of catecholamines. Gen Pharmacol 14:27–33.PubMedCrossRefGoogle Scholar
  7. Halbrügge T, Gerhardt T, Ludwig J, Heidbreder E, Graefe K-H (1988) Assay of catecholamines and dihydroxyphenylethyleneglycol in human plasma and its application in orthostasis and mental stress. Life Sci 43:19–26.PubMedCrossRefGoogle Scholar
  8. Halbrügge T, Wölfel R, Graefe K-H (1989) Plasma 3,4-dihydroxyphenylglycol as a tool to assess the role of neuronal uptake in the anaesthetized rabbit. Naunyn-Schmiedebergs Arch Pharmacol 340:726–732.PubMedGoogle Scholar
  9. Head RJ (1989) Hypernoradrenergic innervation: its relationship to functional and hyperplastic changes in the vasculature of the spontaneously hypertensive rat. Blood Vessels 26:1–20.PubMedGoogle Scholar
  10. Henseling M, Eckert E, Trendelenburg U (1976) The distribution of 3H-(±)-noradrenaline in rabbit aortic strips after inhibition of the noradrenaline-metabolizing enzymes. Naunyn-Schmiedebergs Arch Pharmacol 292:205–217.PubMedCrossRefGoogle Scholar
  11. Kopin IJ (1985) Catecholamine metabolism: basic aspects and clinical significance. Pharmacol Rev 37:333–364.PubMedGoogle Scholar
  12. Kopin IJ, Zukowska-Grojec Z, Bayorh MA, Goldstein DS (1984) Estimation of intrasynaptic norepinephrine concentrations at vascular neuroeffector junctions in vivo. Naunyn-Schmiedebergs Arch Pharmacol 325:298–305.PubMedCrossRefGoogle Scholar
  13. Langer SZ (1974) Selective metabolic pathways for noradrenaline in the peripheral and in the central nervous system. Med Biol 52:372–383.PubMedGoogle Scholar
  14. Ludwig J, Gerhardt T, Haibrügge T, Walter J, Graefe K-H (1988) Plasma concentrations of noradrenaline and 3,4-dihydroxyphenylethyleneglycol under conditions of enhanced sympathetic activity. Eur J Clin Pharmacol 35:261–267.PubMedCrossRefGoogle Scholar
  15. Schultheiss A, Szabo B (1990) Desipramine inhibits sympathetic nerve activity in anaesthetized rabbits. Naunyn-Schmiedebergs Arch Pharmacol 341 [Suppl]: R87.Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K.-H. Graefe
    • 1
    • 2
  • Th. Halbrügge
    • 1
  • M. Gerlich
    • 1
  • J. Ludwig
    • 1
  1. 1.Institut für Pharmakologie und Medizinische PoliklinikUniversity of WürzburgFederal Republic of Germany
  2. 2.Institut für Pharmakologie und ToxikologieUniversität WürzburgWürzburgFederal Republic of Germany

Personalised recommendations