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Biochemical characterization and purification of the neuronal sodium-dependent noradrenaline transporter

  • H. Bönisch
  • G. Martiny-Baron
  • B. Blum
  • J. Michael-Hepp
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)

Summary

The protein properties of the neuronal sodium-dependent noradrenaline (NA) transporter of PC12 (rat pheochromocytoma) cells and of bovine adreno-medullary cells were studied by means of binding of 3H-desipramine (3H-DMI). 3H-DMI binding was decreased by proteases, phospholipase A2, by disulfide reducing agents and by the sulfhydryl-group alkylating agent N-ethylmaleimide. The NA transporter was partially purified by anion exchange and affinity chromatography. Tritiated desmethylxylamine (3H-DMX) bound irreversibly and in a DMI-sensitive manner to two PC12 membrane proteins (32 kd and 53 kd) which may represent components of the NA transporter.

Keywords

PC12 Cell Sodium Cholate PC12 Membrane Sucrose Laurate Noradrenergic Nerve Terminal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • H. Bönisch
    • 1
  • G. Martiny-Baron
    • 1
  • B. Blum
    • 2
  • J. Michael-Hepp
    • 2
  1. 1.Department of Pharmacology, Institut für Pharmakologie und ToxikologieUniversität BonnBonn 1Federal Republic of Germany
  2. 2.Department of PharmacologyUniversity of WürzburgFederal Republic of Germany

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