Biochemical characterization and purification of the neuronal sodium-dependent noradrenaline transporter
The protein properties of the neuronal sodium-dependent noradrenaline (NA) transporter of PC12 (rat pheochromocytoma) cells and of bovine adreno-medullary cells were studied by means of binding of 3H-desipramine (3H-DMI). 3H-DMI binding was decreased by proteases, phospholipase A2, by disulfide reducing agents and by the sulfhydryl-group alkylating agent N-ethylmaleimide. The NA transporter was partially purified by anion exchange and affinity chromatography. Tritiated desmethylxylamine (3H-DMX) bound irreversibly and in a DMI-sensitive manner to two PC12 membrane proteins (32 kd and 53 kd) which may represent components of the NA transporter.
KeywordsPC12 Cell Sodium Cholate PC12 Membrane Sucrose Laurate Noradrenergic Nerve Terminal
Unable to display preview. Download preview PDF.
- Bönisch H, Michael-Hepp J (1989) Binding of 3H-desipramine to the noradrenaline carrier of the plasma membrane of bovine adrenal medulla. Naunyn-Schmiedebergs Arch Pharmacol 340 [Suppl]: R38.Google Scholar
- Graefe K-H, Bönisch H (1988) The transport of amines across the axonal membranes of noradrenergic and dopaminergic neurones. In: Trendelenburg U, Weiner N (eds) Handb exp pharmacol, vol 90/I; Catecholamines I, pp 193-245.Google Scholar
- Iversen LL (1967) The uptake and storage of noradrenaline in sympathetic nerves. Cambridge University Press, Cambridge.Google Scholar
- Michael-Hepp J, Bönisch H (1990) Labelling of the neuronal noradrenaline-carrier with a xylamine derivative. Naunyn-Schmiedebergs Arch Pharmacol 341 [Suppl]: R82.Google Scholar
- Schömig E, Bönisch H (1986) Solubilization and characterization of the 3H-desipramine binding site of rat phaeochromocytoma cells (PC12 cells). Naunyn-Schmiedebergs Arch Pharmacol 37:412–417.Google Scholar