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Monoclonal antibodies recognizing both soluble and membrane bound catechol-O-methyltransferase

  • B. Bertocci
  • G. Garotta
  • G. Zürcher
  • V. Miggiano
  • M. Da Prada
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)

Summary

Both cytosolic, soluble and membrane-bound catechol-O-methyltransferase (COMT) from pig and rat liver or kidney were recognized by mouse monoclonal antibodies (MAbs) raised against soluble COMT isolated from pig liver. In ELISA, the MAbs Co 16 and Co 54 reacted better with the pig than with the rat enzyme. The MAb Co 60 showed good reactivity with both pig and rat COMT. In addition, all three MAbs recognize the soluble (23 kDa) as well as the membrane-bound (26 kDa) forms of the COMT enzyme.

Keywords

Subcellular Fraction Microsomal Fraction Apparent Molecular Mass Microsomal Membrane Good Reactivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • B. Bertocci
    • 1
  • G. Garotta
    • 2
  • G. Zürcher
    • 3
  • V. Miggiano
    • 2
  • M. Da Prada
    • 3
  1. 1.Pharmacological DepartmentUniversity of FlorenceItaly
  2. 2.Central Research DepartmentsF. Hoffmann-La Roche LtdBaselSwitzerland
  3. 3.Pharmaceutical Research DepartmentsF. Hoffmann-La Roche LtdBaselSwitzerland

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