Amine oxidase activities in bovine lung
Part of the
Journal of Neural Transmission
book series (NEURAL SUPPL, volume 32)
The subcellular distributions of amine oxidase activities MAO-A, MAO-B and SSAO, in bovine lung have been determined. MAO activities are largely confined to the mitochondria whereas SSAO activity is concentrated in the microsomal fraction.
The contribution of different type of amine oxidase present in the microsomal fraction to the metabolism of the benzylamine, tyramine, phenylethylamine, tryptamine and dopamine were determined. The kinetic constants towards benzylamine showed SSAO to have a higher affinity than MAO-B, and the comparison of the Vmax/Km relationship for both enzymes indicated that benzylamine would be oxidised more efficiently by SSAO than by MAO-B.
KeywordsBrown Adipose Tissue Microsomal Fraction Amine Oxidase Bovine Lung SSAO Activity
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Bakhle YS, Youdim MBH (1979) The metabolism of 5-hydroxytryptamine and α-phenyl-ethylamine in perfused rat lung and in vitro. Br J Pharmacol 65:147–154.PubMedGoogle Scholar
Barrand MA, Callingham BA (1984) Monoamine oxidase activities in brown adipose tissue of the rat: some properties and subcellular distribution. Biochem Pharmacol 31:2177–2184.CrossRefGoogle Scholar
Elliot J, Callingham BA, Sharman DF (1989) Semicarbazide-sensitive amine oxidase (SSAO) of the rat aorta. Biochem Pharmacol 38:1507–1515.CrossRefGoogle Scholar
Fowler CJ, Tipton KF (1981) Concentration dependence of the oxidation of tyramine by the two forms of rat liver mitochondrial monoamine oxidase. Biochem Pharmacol 30:3329–3332.PubMedCrossRefGoogle Scholar
Roth JA, Gillis CN (1975) Multiple forms of amine oxidases in perfused rabbit lung. J Pharmacol Exp Ther 194:537–544.PubMedGoogle Scholar
Tipton KF (1986) Enzymology of monoamine oxidase. Cell Biol Funt 4:79–87.CrossRefGoogle Scholar