Semicarbazide-sensitive amine oxidase activity in rat aortic cultured smooth muscle cells
Part of the
Journal of Neural Transmission
book series (NEURAL SUPPL, volume 32)
Metabolism of 5 μM benzylamine (BZ) by rat aortic cultured smooth muscle cells (SMC) is inhibited almost completely by 10-3 M semicarbazide and 10-6 M propargylamine, but is little affected by 10-4 M and 10-3 M pargyline and clorgyline, indicating BZ metabolism predominantly by the semicarbazide-sensitive amine oxidase (SSAO) previously characterized in rat aortic homogenates. Km values of 7–9 μM for BZ metabolism by SSAO in SMC cultures, indicate similar, if not identical properties, to the enzyme in the parent blood vessel.
KeywordsSmooth Muscle Cell Amine Oxidase Smooth Muscle Cell Culture Ninewells Hospital Human Umbilical Artery
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