Semicarbazide-sensitive amine oxidase activity in rat aortic cultured smooth muscle cells

  • J. R. D. Blicharski
  • G. A. Lyles
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)


Metabolism of 5 μM benzylamine (BZ) by rat aortic cultured smooth muscle cells (SMC) is inhibited almost completely by 10-3 M semicarbazide and 10-6 M propargylamine, but is little affected by 10-4 M and 10-3 M pargyline and clorgyline, indicating BZ metabolism predominantly by the semicarbazide-sensitive amine oxidase (SSAO) previously characterized in rat aortic homogenates. Km values of 7–9 μM for BZ metabolism by SSAO in SMC cultures, indicate similar, if not identical properties, to the enzyme in the parent blood vessel.


Smooth Muscle Cell Amine Oxidase Smooth Muscle Cell Culture Ninewells Hospital Human Umbilical Artery 
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  1. Hysmith RM, Boor PJ (1988) Role of benzylamine oxidase in the cytotoxicity of allylamine toward aortic smooth muscle cells. Toxicology 51:133–145.PubMedCrossRefGoogle Scholar
  2. Lewinsohn R (1984) Mammalian monoamine-oxidizing enzymes with special reference to benzylamine oxidase in human tissues. Braz J Med Biol Res 17:223–256.PubMedGoogle Scholar
  3. Lyles GA, Singh I (1985) Vascular smooth muscle cells: a major source of the semicarbazide-sensitive amine oxidase of the rat aorta. J Pharm Pharmacol 37:637–643.PubMedCrossRefGoogle Scholar
  4. Precious E, Gunn CE, Lyles GA (1988) Deamination of methylamine by semicarbazide-sensitive amine oxidase in human umbilical artery and rat aorta. Biochem Pharmacol 37:707–713.PubMedCrossRefGoogle Scholar
  5. Ramos K, Grossman SL, Cox LR (1988) Allylamine-induced vascular toxicity in vitro: prevention by semicarbazide-sensitive amine oxidase inhibitors. Toxicol Appl Pharmacol 95:61–71.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • J. R. D. Blicharski
    • 1
  • G. A. Lyles
    • 1
  1. 1.Department of Pharmacology and Clinical PharmacologyUniversity of Dundee, Ninewells Hospital and Medical SchoolDundeeUK

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