An enzymatic method for detecting MAO-A and MAO-B inhibitors in plasma and its application in studies with reversible MAO-A selective inhibitors

  • Helen L. White
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)


A general method was developed for the simultaneous enzymatic assay of MAO-A and MAO-B inhibitors in plasma of pretreated animals. After extraction of the inhibitor from plasma into an appropriate volatile organic solvent, dried aliquots of the organic layer are incubated with a buffered rat brain MAO preparation and substrates, [3H]serotonin and [14C]phenethylamine. Under conditions employed, the presence in plasma of MAO-A and/or B inhibitors can be detected.


Butyl Ether Pretreated Animal Specific HPLC Typical Standard Curve Specific HPLC Method 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Helen L. White
    • 1
  1. 1.Division of PharmacologyWellcome Research LaboratoriesResearch Triangle ParkUSA

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