Summary
A general method was developed for the simultaneous enzymatic assay of MAO-A and MAO-B inhibitors in plasma of pretreated animals. After extraction of the inhibitor from plasma into an appropriate volatile organic solvent, dried aliquots of the organic layer are incubated with a buffered rat brain MAO preparation and substrates, [3H]serotonin and [14C]phenethylamine. Under conditions employed, the presence in plasma of MAO-A and/or B inhibitors can be detected.
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References
Fritze J, Laux G, Sofic E, Koronakis P, Schoerlin MP, Riederer P, Beckmann H (1989) Plasma moclobemide and metabolites: lack of correlation with clinical response and biogenic amines. Psychopharmacology 99:252–256.
White HL, Stine DK (1982) Monoamine oxidases A and B as components of a membrane complex. J Neurochem 38:1429–1436.
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© 1990 Springer-Verlag
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White, H.L. (1990). An enzymatic method for detecting MAO-A and MAO-B inhibitors in plasma and its application in studies with reversible MAO-A selective inhibitors. In: Riederer, P., Youdim, M.B.H. (eds) Amine Oxidases and Their Impact on Neurobiology. Journal of Neural Transmission, vol 32. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9113-2_20
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DOI: https://doi.org/10.1007/978-3-7091-9113-2_20
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82239-5
Online ISBN: 978-3-7091-9113-2
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