[3H] Harman labels selectively and with high affinity the active site of monoamine oxidase (EC subtype A (MAO-A) in rat, marmoset, and pig

  • T. May
  • S. Strauss
  • H. Rommelspacher
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 32)


[3H]Harman was used in binding studies with CNS tissue of rat, pig, and marmoset and with visceral organs of the rat.

In the mitochondrial fractions of the CNS of the 3 species [3H]harman binding exhibits the same pharmacological profile in displacement studies. A detailed analysis reveals a high specificity for MAO-A.

Furthermore, we applied [3H]harman binding to quantify the MAO-A content in 6 CNS regions of each species as well as in 6 visceral organs of the rat.


Monoamine Oxidase Visceral Organ Scatchard Analysis Bovine Serum Albumine Crude Mitochondrial Fraction 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • T. May
    • 1
  • S. Strauss
    • 1
  • H. Rommelspacher
    • 1
  1. 1.Department of NeuropsychopharmacologyFree UniversityBerlin 19Federal Republic of Germany

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