The Manipulation of Plant Gene Expression Using Antisense RNA
Gene cloning techniques have allowed us to analyse in considerable detail the structure of plant genes. The availability of probes for plant genes has opened the way for detailed studies on the temporal and spatial expression of plant genes during the development of different plant organs and tissues (Kuhlemeier et al., 1987). Functional analysis of isolated plant genes was made possible through the development of gene transfer techniques (Weising et al., 1988). This has led to the detailed description of expression patterns exhibited by gene promoter sequences, and to the delineation of DNA sequences which are required for the temporal and spatial control of gene expression.
KeywordsChloramphenicol Acetyl Transferase Antisense Gene Plant Gene Expression Chloramphenicol Acetyl Transferase Activity Phosphinothricin Acetyl Transferase
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