Hemorrhagic fever with renal syndrome in Bulgaria: isolation of hantaviruses and epidemiologic considerations
Epidemiological analysis of the incidence of hemorrhagic fever with renal syndrome, 1953–1988, showed that this infection is prevalent in many areas of Bulgaria. We developed a method ensuring 100% etiological diagnosis of the diseases by using albino mice for virus isolation and preparation of antigens for serological studies using a complement-fixation test. Experimental infection with hantaviruses was produced in newborn white mice. In these hosts, lesions were mostly observed in the brain, with considerable accumulation of virus detectable by electron microscopic and fluorescent antibody techniques. The virus of hemorrhagic fever with renal syndrome was successfully adapted to cell culture, in which it produced distinct cytopathic effects.
KeywordsBank Vole Hemorrhagic Fever With Renal Syndrome Viral Encephalitis Natural Focus Dilate Blood Vessel
Unable to display preview. Download preview PDF.
- 1.Gavrilovaskaya I, Vasilenko S, Chumakov M, Shindarov L, Katsarov G (1984) HFRS in Bulgaria: prevalence and serological evidence. Epidemiol Microbiol Infect Dis (Sofia) 4: 17–23Google Scholar
- 3.Glick A, Frusiè M, Obradovic M, Stojanovic R, Hlaca D, Gibbs CJ Jr, Yanagihara R, Calisher CH, Gajdusek DC (1989) Hemorrhagic fever with renal syndrome in Yugoslavia: antigenic characterization of hantaviruses isolated from Apodemus flavicollis and Clethrionomys glareolus. Am J Trop Med Hyg 41: 109–115.Google Scholar
- 4.Tchumakov M, Shindarov L, Gavrilovskaya I, Vasilenko S, Gorbachkova E, Katsarov G (1988) Seroepidemiology of HFRS in Bulgaria. Acta Virol 32: 261–266Google Scholar
- 5.Vasilenko S, Bradvarova I (1972) On the results of investigation of HFRS etiology in Bulgaria. In: Aktualnye problemy virusologii i profilaktiki virusnykh zabolevaniy. Abstracts 17th scientific session of the Institute of Poliomyelitis and Viral Encephalitis, Moscow, October 24–27, 1972, p 304Google Scholar