Field trial of an inactivated vaccine against hemorrhagic fever with renal syndrome in humans
Hantaan virus was serially passaged in the brains of suckling rats. Vaccine inactivated with formalin was prepared from the suckling rat brains and the antigenic potency of the vaccine was determined by enzyme-linked immunosorbent assay (ELISA). Mice immunized with the vaccine were protected when challenged with live Hantaan virus. The antibody responses of 456 vaccinees given inactivated Hantaan virus vaccine against hemorrhagic fever with renal syndrome (HFRS) were then studied. Subcutaneous injection of the vaccine was better than intramuscular injection for production of antibodies in humans. Optimal immunogenic dose of the vaccine given subcutaneously to humans was 5,120 ELISA antigen units. Of 456 vaccinees, immunization of 336 with two doses of vaccine at one month interval resulted in 99% seroconversion by indirect immunofluorescent antibody tests. The vaccine was safe and only minimum side effects were observed. The efficacy of this vaccine against HFRS in the endemic areas of HFRS remains to be determined.
KeywordsJapanese Encephalitis Japanese Encephalitis Hemorrhagic Fever With Renal Syndrome Seroconversion Rate Vaccine Preparation
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