Rapid serodiagnosis of hantavirus infections using high density particle agglutination

  • Tetsuo Tomiyama
  • H. W. Lee
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 1)


Antibody against hantaviruses was measured within forty minutes by a passive agglutination procedure using high density composite particles coated with purified Hantaan virus antigen. Antigen for the reaction was prepared from the brains of suckling rats infected with Hantaan virus, using ultracentrifugation, protamine and ethyl alcohol treatment. This method is more sensitive than the immunofluorescent antibody technique and the antigen reacted with antibodies to Hantaan, Seoul and Puumala viruses.


Hemorrhagic Fever With Renal Syndrome Rift Valley Fever Hantavirus Infection Nephropathia Epidemica Hantaan Virus 
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  1. 1.
    Lee HW, Lee PW, Johnson KM (1978) Isolation of the etiologic agent of Korean hemorrhagic fever. J Infect Dis 137: 298–308PubMedCrossRefGoogle Scholar
  2. 2.
    Lee HW (1988) Global update on distribution of hantaviruses and hemorrhagic fever with renal syndrome. Virus Info Exch Newslett 5: 82–84Google Scholar
  3. 3.
    Lee HW, An CN (1988) Development of a vaccine against hemorrhagic fever with renal syndrome. J Kor Soc Virol 18: 143–148Google Scholar
  4. 4.
    Lee HW (1989) Hemorrhagic fever with renal syndrome in Korea. Rev Infect Dis 2: S864 – S876Google Scholar
  5. 5.
    Meegan JM, LeDuc JW (1989) Enzyme immunoassays. In: Lee HW, Dalrymple JM (eds) Manual of hemorrhagic fever with renal syndrome. WHO Collaborating Centre for Virus Reference and Research (HFRS), Institute for Viral Diseases, Korea University, Seoul, pp 83–87Google Scholar
  6. 6.
    Mitani K, Une H (1988) Dyed inorganic composite particles and process for production thereof. U.S. Patent 4,780, 422Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Tetsuo Tomiyama
    • 1
  • H. W. Lee
    • 2
  1. 1.Laboratory of Microbiology and ImmunologyUniversity of Tokyo Branch HospitalBunkyo-ku, Tokyo 112Japan
  2. 2.The Institute for Viral DiseasesKorea UniversitySeoulKorea

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