Advertisement

Isolation of Crimean-Congo hemorrhagic fever virus from patients and from autopsy specimens

  • A. M. Butenko
  • M. P. Chumakov
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 1)

Summary

We tested 57 blood specimens from 51 Crimean-Congo hemorrhagic fever (CCHF) patients and organ tissue specimens from three autopsy cases for virus isolation. Blood specimens were inoculated intracerebrally into newborn albino mice within 20 min after collection or stored them at 4 °C for no more than 10 days before inoculation. Viremia was observed within the first seven days of the disease in almost all CCHF patients and the virus often was demonstrable in the blood for 8–12 days after onset of illness. Attempts to isolate virus from urine samples of patients were ineffective. Maximum virus titers (to 6.2 log10 LD50/m1) were present in patients’ blood for the first five days of illness. CCHF virus was detected in hypothalamus, bone marrow, breast lymphatic glands, kidney, adrenals, and in the large intestine wall of autopsy specimens.

Keywords

Virus Isolation Hemorrhagic Fever Autopsy Specimen Viral Encephalitis Rostov Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Al-Tikriti SK, Al-Ami F, Jurji FJ, Tantavi H, Al-Moslih M, Al-Janabi N, Mahmud MJA, Al-Bana A, Habid H, et al (1981) Congo-Crimean hemorrhagic fever in Iraq. Bull WHO 59: 1: 85–90PubMedGoogle Scholar
  2. 2.
    Burney MI, Ghafoor A, Saleen M, Webb PA, Casals J (1980) Nosocomial outbreak of viral hemorrhagic fever caused by Crimean-Congo virus in Pakistan, January 1976. Am J Trop Med Hyg 29: 941–947PubMedGoogle Scholar
  3. 3.
    Butenko AM (1971) Data from studying etiology, laboratory diagnosis and immunology of Crimean hemorrhagic fever; questions of ecology of the viral agent. Avtoref Diss Soisk Uchen Step Dokt Biol Nauk Inst Poliomiel Virus Encephal Acad Med Nauk SSSR, Moscow, (in Russian) (in English, NAMRU3-T-1152)Google Scholar
  4. 4.
    Butenko AM, Chumakov MP, Bashkirtsev VN, Zavodova TI, Tkachenko EA, Rubin SG, Stolbov DN (1968) Isolation and investigation of Astrakhan strain (“Drozdov”) of Crimean hemorrhagic fever virus and data on serodiagnosis of this infection. Mater 15 Nauchn Sess Inst Poliomiel Virus Encephal Acad Med Nauk SSSR (October 1968) 3: 88–90 (in Russian) (in English, NAMRU3-T-866)Google Scholar
  5. 5.
    Casey HL (1965) Standardized diagnostic complement fixation method and adaptation to micro test, part II. In: Pub Health Monogr No 74; Adaptation of LBCF method to micro technique, pp 31–34 (P.H.S. Publication No 1228 ) US Government Printing Office, Washington, DCGoogle Scholar
  6. 6.
    Leshchinskaya EV (1967) Clinical picture of Crimean hemorrhagic fever and its comparison with hemorrhagic fevers of other types. Avtoref Diss Soisk Uchen Step Dokt Med Nauk Inst Poliomiel Encephal Acad Med Nauk SSSR, Moscow (in Russian) (in English, NAMRU3-T-1180)Google Scholar
  7. 7.
    Murphy FA, Coleman PH (1967) California group arboviruses: immunodiffusion studies. J Immunol 99: 276–284PubMedGoogle Scholar
  8. 8.
    Ouchterlony O (1958) Diffusion in gel methods for immunological analysis. Prog Allergy 5: 1–78PubMedGoogle Scholar
  9. 9.
    Shepherd AI, Swanepoel R, Shepherd SP, Leman PA, Blackburn NK, Haller AF (1985) A nosocomial outbreak of Crimean-Congo hemorrhagic fever at Tygerberg Hospital. V. Virological and serological observations. S Afr Med J 68: 733–736PubMedGoogle Scholar
  10. 10.
    Tikasingh ES, Spence L, Downs WG (1966) The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune fluids to arboviruses. Am J Trop Med Hyg 15: 219–226PubMedGoogle Scholar
  11. 11.
    Vasilenko SM (1973) Results of the investigation on etiology, epidemiologic features, and the specific prophylaxis of Crimean hemorrhagic fever in Bulgaria. In: Proc 9 Int Congr Trop Med Malaria, Athens, Greece 1: 32–33Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. M. Butenko
    • 1
  • M. P. Chumakov
    • 2
  1. 1.Department of Virus Ecology, D.I. Ivanovsky Institute of VirologyAcademy of Medical Sciences of the U.S.S.R.MoscowUSSR
  2. 2.Institute of Poliomyelitis and Viral EncephalitidesU.S.S.R. Academy of Medical SciencesMoscowUSSR

Personalised recommendations