Isolation of Crimean-Congo hemorrhagic fever virus from patients and from autopsy specimens
We tested 57 blood specimens from 51 Crimean-Congo hemorrhagic fever (CCHF) patients and organ tissue specimens from three autopsy cases for virus isolation. Blood specimens were inoculated intracerebrally into newborn albino mice within 20 min after collection or stored them at 4 °C for no more than 10 days before inoculation. Viremia was observed within the first seven days of the disease in almost all CCHF patients and the virus often was demonstrable in the blood for 8–12 days after onset of illness. Attempts to isolate virus from urine samples of patients were ineffective. Maximum virus titers (to 6.2 log10 LD50/m1) were present in patients’ blood for the first five days of illness. CCHF virus was detected in hypothalamus, bone marrow, breast lymphatic glands, kidney, adrenals, and in the large intestine wall of autopsy specimens.
KeywordsVirus Isolation Hemorrhagic Fever Autopsy Specimen Viral Encephalitis Rostov Region
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