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New perspective vaccines from tick-borne encephalitis virus propagated in green monkey kidney cell cultures

  • M. P. Chumakov
  • S. G. Rubin
  • I. V. Semashko
  • M. N. Matrosovich
  • L. L. Mironova
  • L. I. Martyanova
  • Yu. A. Kniaginskaya
  • Ya. A. Salnikov
  • A. S. Gambaryan
  • A. S. Karavanov
  • O. V. Kurennaya
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 1)

Summary

A new inactivated absorbed tick-borne encephalitis (TBE) vaccine from TBE virus propagated in primary green monkey kidney cells (MKC) is offered. The utilization of this cellular substrate provides a stable high accumulation of the virus in consecutive harvests of culture fluid made at 2–3-day intervals and results in the following advantages of the new TBE vaccine:
  • abundance of a cheap virus source for vaccine production;

  • an absence of necessity to concentrate the virus;

  • considerable lowering of content of total protein and host-derived impurities;

  • the high specific seroconversion and non-reactogenicity of the vaccine as judged by observation of volunteers.

The technology of TBE vaccine production in MKC-derived continuous cell line 4647 using simple cellular DNA-decontamination procedure was also developed.

The advantages of the technology used for preparation of this TBE vaccine could be employed for production of vaccines against other viral infections.

Keywords

Vaccine Production Viral Encephalitis Monkey Kidney Cell Continuous Cell Line Neutralization Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • M. P. Chumakov
    • 1
    • 2
  • S. G. Rubin
    • 2
  • I. V. Semashko
    • 2
  • M. N. Matrosovich
    • 2
  • L. L. Mironova
    • 2
  • L. I. Martyanova
    • 2
  • Yu. A. Kniaginskaya
    • 2
  • Ya. A. Salnikov
    • 2
  • A. S. Gambaryan
    • 2
  • A. S. Karavanov
    • 2
  • O. V. Kurennaya
    • 2
  1. 1.Institute of Poliomyelitis and Viral Encephalitides of the U.S.S.R.Academy of Medical SciencesMoscow RegionUSSR
  2. 2.Institute of Poliomyelitis and Viral EncephalitidesU.S.S.R. Academy of Medical ScienceMoscowUSSR

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