Summary
A new inactivated absorbed tick-borne encephalitis (TBE) vaccine from TBE virus propagated in primary green monkey kidney cells (MKC) is offered. The utilization of this cellular substrate provides a stable high accumulation of the virus in consecutive harvests of culture fluid made at 2–3-day intervals and results in the following advantages of the new TBE vaccine:
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abundance of a cheap virus source for vaccine production;
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an absence of necessity to concentrate the virus;
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considerable lowering of content of total protein and host-derived impurities;
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the high specific seroconversion and non-reactogenicity of the vaccine as judged by observation of volunteers.
The technology of TBE vaccine production in MKC-derived continuous cell line 4647 using simple cellular DNA-decontamination procedure was also developed.
The advantages of the technology used for preparation of this TBE vaccine could be employed for production of vaccines against other viral infections.
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References
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© 1990 Springer-Verlag
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Chumakov, M.P. et al. (1990). New perspective vaccines from tick-borne encephalitis virus propagated in green monkey kidney cell cultures. In: Calisher, C.H. (eds) Hemorrhagic Fever with Renal Syndrome, Tick- and Mosquito-Borne Viruses. Archives of Virology Supplementum, vol 1. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9091-3_19
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DOI: https://doi.org/10.1007/978-3-7091-9091-3_19
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82217-3
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