A study of the NS3 nonstructural protein of tick-borne encephalitis virus using monoclonal antibodies against the virus
We obtained two monoclonal antibodies against native NS3 nonstructural protein of tick-borne encephalitis (TBE) virus, strain Sofyin. Using these monoclonal antibodies, we were able to determine that NS3 is a stable protein with preferential perinuclear localization. Both monoclonal antibodies precipitate NS3 proteins of TBE strains Sofyin and Absettarov and of Langat virus but do not precipitate analogous proteins of other flaviviruses (Powassan, yellow fever, Japanese encephalitis). Taking into account the work of others, we suggest that there are at least two distinct epitopes on NS3 protein. A putative functional model of NS3 is discussed.
KeywordsWest Nile Virus Yellow Fever Japanese Encephalitis Japanese Encephalitis Virus Yellow Fever Virus
Unable to display preview. Download preview PDF.
- 4.Gorbalenya AE, Donchenko AP, Koonin EV, Blinov VM (1989) N-terminal domains of putative helicases of flavi-and pestiviruses may be serine proteases. Nucleic Acids Res 17: 3879–3897Google Scholar
- 8.Kushch AA, Novak M, Melnikova YeF, Gaidamovich SY, Gresikova M, Sekeyova M, Novokhatsky AS, Mikheeva TG, Sveshnikova NA, Borecky LS, Zhdanov VM (1986) Preparation and characterization of hybridomas secreting monoclonal antibodies to tick-borne encephalitis virus. Acta Virol 30: 199–205PubMedGoogle Scholar
- 12.O’Farrel PH (1975) High-resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007–4021Google Scholar