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A study of the NS3 nonstructural protein of tick-borne encephalitis virus using monoclonal antibodies against the virus

  • A. V. Timofeev
  • Alla A. Kushch
  • M. F. Vorovitch
  • S. M. Tugizov
  • L. B. Elbert
  • D. K. Lvov
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 1)

Summary

We obtained two monoclonal antibodies against native NS3 nonstructural protein of tick-borne encephalitis (TBE) virus, strain Sofyin. Using these monoclonal antibodies, we were able to determine that NS3 is a stable protein with preferential perinuclear localization. Both monoclonal antibodies precipitate NS3 proteins of TBE strains Sofyin and Absettarov and of Langat virus but do not precipitate analogous proteins of other flaviviruses (Powassan, yellow fever, Japanese encephalitis). Taking into account the work of others, we suggest that there are at least two distinct epitopes on NS3 protein. A putative functional model of NS3 is discussed.

Keywords

West Nile Virus Yellow Fever Japanese Encephalitis Japanese Encephalitis Virus Yellow Fever Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bazan JF, Fletterick RJ (1989) Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology 171: 637–639PubMedCrossRefGoogle Scholar
  2. 2.
    Coia G, Perker MD, Speight G, Byrne ME, Westaway EG (1988) Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified protein. J Gen Virol 69: 1–21PubMedCrossRefGoogle Scholar
  3. 3.
    Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM (1989) Two related super-families of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res 17: 4713–4730PubMedCrossRefGoogle Scholar
  4. 4.
    Gorbalenya AE, Donchenko AP, Koonin EV, Blinov VM (1989) N-terminal domains of putative helicases of flavi-and pestiviruses may be serine proteases. Nucleic Acids Res 17: 3879–3897Google Scholar
  5. 5.
    Gould EA, Chanar AC, Buckley A, Clegg CS (1983) Monoclonal immunoglobulin M antibody to Japanese encephalitis virus that can react with a nuclear antigen in mammalian cells. Infect Immun 41: 774–779PubMedGoogle Scholar
  6. 6.
    Grun JB, Brinton MA (1986) Characterization of West Nile virus RNA-dependent RNA polymerase and cellular terminal adenylyl and uridylyl transferases in cell-free extracts. J Virol 60: 1113–1124PubMedGoogle Scholar
  7. 7.
    Hahn YS, Galler R, Hunkapiller T, Dalrymple JM, Strauss SH, Strauss EG (1988) Nucleotide sequence of dengue 2 RNA and comparison of the encoded proteins with those of other flaviviruses. Virology 162: 167–180PubMedCrossRefGoogle Scholar
  8. 8.
    Kushch AA, Novak M, Melnikova YeF, Gaidamovich SY, Gresikova M, Sekeyova M, Novokhatsky AS, Mikheeva TG, Sveshnikova NA, Borecky LS, Zhdanov VM (1986) Preparation and characterization of hybridomas secreting monoclonal antibodies to tick-borne encephalitis virus. Acta Virol 30: 199–205PubMedGoogle Scholar
  9. 9.
    Lyapustin VN, Gritsun TS, Lashkevich VA (1986) Effect of multiplicity of infection on synthesis of the tick-borne encephalitis virus specific proteins during a single reproduction cycle. Acta Virol 30: 289–293PubMedGoogle Scholar
  10. 10.
    Ng ML, Hong SS (1989) Flavivirus infection: essential ultrastructural changes and association of Kunjin virus NS3 protein with microtubules. Arch Virol 106: 103–120PubMedCrossRefGoogle Scholar
  11. 11.
    Ng ML, Corner LC (1989) Detection of some dengue-2 virus antigens in infected cells using immunomicroscopy. Arch Virol 104: 197–208PubMedCrossRefGoogle Scholar
  12. 12.
    O’Farrel PH (1975) High-resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007–4021Google Scholar
  13. 13.
    Rice CM, Lenches EM, Eddy SR, Shin SJ, Sheets RL, Strauss JH (1985) Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229: 726–733PubMedCrossRefGoogle Scholar
  14. 14.
    Stephenson JR, Grooks AJ, Lee JM (1987) The synthesis of immunogenic polypeptides encoded by tick-borne encephalitis virus. J Gen Virol 68: 1307–1316PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. V. Timofeev
    • 1
    • 3
  • Alla A. Kushch
    • 2
  • M. F. Vorovitch
    • 1
  • S. M. Tugizov
    • 2
  • L. B. Elbert
    • 1
  • D. K. Lvov
    • 2
  1. 1.Institute of Poliomyelitis and Viral EncephalitidesMoscowUSSR
  2. 2.D.I. Ivanovsky Institute of VirologyMoscowUSSR
  3. 3.Institute of Poliomyelitis and Viral EncephalitidesU.S.S.R. Academy of Medical SciencesMoscowUSSR

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