A study of the NS3 nonstructural protein of tick-borne encephalitis virus using monoclonal antibodies against the virus

  • A. V. Timofeev
  • Alla A. Kushch
  • M. F. Vorovitch
  • S. M. Tugizov
  • L. B. Elbert
  • D. K. Lvov
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 1)


We obtained two monoclonal antibodies against native NS3 nonstructural protein of tick-borne encephalitis (TBE) virus, strain Sofyin. Using these monoclonal antibodies, we were able to determine that NS3 is a stable protein with preferential perinuclear localization. Both monoclonal antibodies precipitate NS3 proteins of TBE strains Sofyin and Absettarov and of Langat virus but do not precipitate analogous proteins of other flaviviruses (Powassan, yellow fever, Japanese encephalitis). Taking into account the work of others, we suggest that there are at least two distinct epitopes on NS3 protein. A putative functional model of NS3 is discussed.


West Nile Virus Yellow Fever Japanese Encephalitis Japanese Encephalitis Virus Yellow Fever Virus 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. V. Timofeev
    • 1
    • 3
  • Alla A. Kushch
    • 2
  • M. F. Vorovitch
    • 1
  • S. M. Tugizov
    • 2
  • L. B. Elbert
    • 1
  • D. K. Lvov
    • 2
  1. 1.Institute of Poliomyelitis and Viral EncephalitidesMoscowUSSR
  2. 2.D.I. Ivanovsky Institute of VirologyMoscowUSSR
  3. 3.Institute of Poliomyelitis and Viral EncephalitidesU.S.S.R. Academy of Medical SciencesMoscowUSSR

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