Phenolsulfotransferase (PST) and PAPS in catecholamine metabolism in human tissues: an overview

  • K. P. Wong
  • B. Y. Khoo
  • T. M. C. Tan
  • K. H. Sit
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 29)


Sulfate conjugation in vitro was studied in a number of human tissues, such as the platelets, liver as well as in human foetal liver cells in culture. Essentially, the two enzymes involved in PAPS generation and PST were measured either singly or in combination, representing PAPS generation, PST and the overall three-step sulfate conjugation. These activities in the liver and platelets were comparable in magnitude. In the platelets, PAPS generation and PST activities showed a good correlation to the overall sulfate conjugation. With the expression of these activities in human foetal liver cells in culture, it is anticipated that such cells can be employed as model systems for the study of sulfate conjugation in man.


Human Liver Biogenic Amine Human Platelet Sulfate Conjugation Extrahepatic Tissue 
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  1. Anderson RJ, Weinshilboum RM (1980) Phenolsulfotransferase in human tissues: radio-chemical enzymatic assay and biochemical properties. Clin Chim Acta 103: 79–90.PubMedCrossRefGoogle Scholar
  2. Baranczyk-Kuzma, Szymczyk T (1986) Lung phenolsulfotransferases: thermal stability of human and bovine enzymes. Biochem Pharmacol 35: 995–999.PubMedCrossRefGoogle Scholar
  3. Campbell NRC, Van Loon JA, Weinshilboum RM (1987) Human liver phenol sulfotrans-ferase: assay conditions, biochemical properties and partial purification of isozymes of the thermostable form. Biochem Pharmacol 36: 1435–1446.PubMedCrossRefGoogle Scholar
  4. Elchisak MA, Hausner EA (1984) Demonstration of N-acetyldopamine in human kidney and urine. Life Sci 35: 2561–2569.PubMedCrossRefGoogle Scholar
  5. Foldes A, Meek JL (1973) Rat brain phenolsulphotransferase-partial purification and some properties. Biochem Biophys Acta 327: 365–375.PubMedGoogle Scholar
  6. Hart RF, Rensker KJ, Nelson EB, Roth JA (1979) Localization and characterization of phenol sulfotransferase in human platelets. Life Sci 24: 125–130.PubMedCrossRefGoogle Scholar
  7. Johnson GA, Baker CA, Smith RT (1980) Radioenzymatic assay of sulfate conjugates of catecholamines and dopa in plasma. Life Sci 26: 1591–1598.PubMedCrossRefGoogle Scholar
  8. Kahane Z, Esser AH, Kline NS, Vestergaard P (1967) Estimation of conjugated epinephrine and norepinephrine in urine. J Lab Clin Med 69: 1042–1050.PubMedGoogle Scholar
  9. Khoo BY, Sit KH, Wong KP (1988 a) The human platelet as an independent unit for sulfate conjugation. Life Sci 42: 1165–1171.PubMedCrossRefGoogle Scholar
  10. Khoo BY, Sit KH, Wong KP (1988 b) Does PAPS generation determine the overall sulfate conjugation of human platelets? Life Sci 42: 2389–2395.PubMedCrossRefGoogle Scholar
  11. Khoo BY, Wong KP (1988c) Kinetic studies of PAPS-generation and overall sulfate conjugation in human liver. 14th IUB Congress, Prague, Czechoslovakia, 10–15 July 1988, vol 4 (abstract 613), pp 217.Google Scholar
  12. Picotti GB, Cesura AM, Galva MD, Mantegazza P, Kettler R, Da Prada M (1981) Mono-amine sulfoconjugation by human platelets. In: Sandier M, Usdin E (eds) Phenolsul-fotransferase in mental health research. Macmillan, London Basingstoke, pp 44–54.Google Scholar
  13. Rein G, Glover V, Sandier M (1981) Phenolsulfotransferase in human tissue: evidence for multiple forms. In: Sandier M, Usdin E (eds) Phenolsulfotransferase in mental health research. Macmillan, London Basingstoke, pp 98–126.Google Scholar
  14. Rein G, Glover V, Sandier M (1982) Multiple forms of phenolsulphotransferase in human tissue. Selective inhibition by dichloronitrophenol. Biochem Pharmacol 31: 1893–1897.PubMedCrossRefGoogle Scholar
  15. Rein G, Glover V, Sandier M (1984) Characterization of human brain phenolsulphotransferase. J Neurochem 42: 80–85.PubMedCrossRefGoogle Scholar
  16. Renskers KJ, Feor KD, Roth JA (1980) Sulfation of dopamine and other biogenic amines by human brain phenol sulfotransferase. J Neurochem 34: 1362–1368.PubMedCrossRefGoogle Scholar
  17. Richter D (1940) The inactivation of adrenaline in vitro in man. J Physiol (London) 98: 361–374.Google Scholar
  18. Rivett AJ, Eddy BJ, Roth JA (1982) Contribution of sulfate conjugation, deamination and O-methylation to metabolism of dopamine and norepinephrine in human brain. J Neurochem 39: 1009–1016.PubMedCrossRefGoogle Scholar
  19. Roth JA, Rivett AJ, Renskers KJ (1982) Properties of human brain phenolsulfotransferase and its role in the inactivation of catecholamine neurotransmitters. In: Mulders GJ, et al (eds) Sulfate metabolism and sulfate conjugation. Taylor and Francis, pp 107-114.Google Scholar
  20. Steiner E, von Bahr C, Rane A (1982) Sulphate and glucuronic acid conjugation of harmol in human fetal and adult liver tissue. Dev Pharmacol Ther 5: 14–20.PubMedGoogle Scholar
  21. Tan TMC, Sit KH, Wong KP (1988 a) Sulfate conjugation by human foetal liver cells in culture. 14th IUB Congress, Prague, Czechoslovakia, 10–15 July 1988, vol 4 (abstract 612), pp216.Google Scholar
  22. Tan TMC, Sit KH, Wong KP (1988 b) Kinetic studies of sulphate conjugation in extracts of human foetal cells in culture. Biochem Pharmacol 24: 4629–4633.CrossRefGoogle Scholar
  23. Toth LA, Elchisak MA (1987) Pharmacological characterization of dopamine sulfoconjugation by human platelets. J Pharmacol Exp Ther 240: 359–363.PubMedGoogle Scholar
  24. Tyce GM, Rorie DK, Byer DE, Danielson DR (1985) Free and conjugated amines in human lumbar cerebrospinal fluid. J Neurochem 44: 322–324.PubMedCrossRefGoogle Scholar
  25. Tyce GM, Messick JM, Yaksh TL, Byer DE, Danielson DR, Rorie DK (1986) Amine sulfate formation in the central nervous system. Fed Proc 45: 2247–2253.PubMedGoogle Scholar
  26. Tyce GM, Van Dyke RA, Rettke SR, Atchison SR, Wiesner RH, Dickson ER, Krom RA (1987) Human liver and conjugation of catecholamines. J Lab Clin Med 109: 532–537.PubMedGoogle Scholar
  27. Weil-Malherbe H, Bone AD (1958) The association of adrenaline and noradrenaline with blood platelets. Biochem J 70: 14–22.PubMedGoogle Scholar
  28. Whittemore RM, Pearce B, Roth JA (1985) Purification and kinetic characterization of a dopamine-sulfating form of phenol sulfotransferase from human brain. Biochemistry 24: 2477–2482.PubMedCrossRefGoogle Scholar
  29. Wong KP (1976) The conjugation of tyramine with sulphate by liver and intestine of different animals. Biochem J 160: 491–493.PubMedGoogle Scholar
  30. Wong KP (1978) Measurement and kinetic study of the formation of adrenaline sulphate in vitro. Biochem J 174: 777–782.PubMedGoogle Scholar
  31. Wong KP, Yeo T (1979) Assay of adenosine 3′-phosphate 5′-sulphatophosphate in hepatic tissues. Biochem J 181: 107–110.PubMedGoogle Scholar
  32. Wong KP (1981) A comparison of the PAPS-generating systems of various tissues by a simple fluorimetric procedure. Asian J Pharmac Sci 3: 3–5.Google Scholar
  33. Wong KP (1982a) Sulfate conjugation of amines and their metabolites. In: Mulders GJ, et al (eds) Sulfate metabolism and sulfate conjugation. Taylor and Francis, pp 85-92.Google Scholar
  34. Wong KP (1982 b) Sulphate conjugation of isoprenaline by lung, small intestine and other organs. Biochem Pharmacol 31: 59–62.PubMedCrossRefGoogle Scholar
  35. Wong KP, Yeo T (1982) Importance of extrahepatic sulphate conjugation. Biochem Pharmacol 31: 4001–4003.PubMedCrossRefGoogle Scholar
  36. Wong KP, Yeo T, Tsoi WF (1984) Human platelet phenolsulfotransferase (PST) activity in normal and schizophrenic subjects. Biogenic Amines 1: 319–328.Google Scholar
  37. Wong KP, Wong KF (1985) Sulfate conjugation in human liver and lung. Biogenic Amines 2: 93–100.Google Scholar
  38. Wong KP, Yeo T, Rauff A (1985) Sulfate conjugation of biogenic amines by human omentum. Biogenic Amines 2: 73–81.Google Scholar
  39. Wong KP, Ho BC (1986) Phenolsulphotransferase (PST) activity in the developing mosquito (Aedes togoi). Insect Biochem 17: 111–116.Google Scholar
  40. Wong KP, Khoo BY (1986) Sulfate conjugation of N-acetyldopamine (NADA) by human platelet phenolsulfotransferase (PST). In: Kon et al (eds) ICSU Short reports, vol 6: Contemporary themes in biochemistry. Cambridge University Press, pp 78-79.Google Scholar
  41. Wong KP (1987) N-acetyldopamine, a good substrate of rat brain phenolsulfotransferase. Biogenic Amines 4: 5–16.Google Scholar
  42. Yoneda S, Alexander N, Vlachakis ND (1984) Conjugated normetanephrine in human and rat plasma and erythrocytes. Biochem Pharmacol 33: 2029–2032.PubMedCrossRefGoogle Scholar
  43. Young WF, Okazaki H, Laws ER, Weinshilboum RM (1984) Human brain phenol sul-fotransferase: biochemical properties and regional localization. J Neurochem 43: 706–715.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K. P. Wong
    • 1
    • 3
  • B. Y. Khoo
    • 1
  • T. M. C. Tan
    • 1
  • K. H. Sit
    • 2
  1. 1.Department of Biochemistry, Faculty of MedicineNational University of SingaporeSingapore
  2. 2.Department of Anatomy, Faculty of MedicineNational University of SingaporeSingapore
  3. 3.Department of Biochemistry, Faculty of MedicineNational University of SingaporeSingapore 0511Singapore

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